Pulmonary heat shock protein expression after exposure to a metabolically activated Clara cell toxicant: Relationship to protein adduct formation

Kurt J. Williams, Michael K. Cruikshank, Charles Plopper

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Heat shock proteins/stress proteins (Hsps) participate in regulation of protein synthesis and degradation and serve as general cytoprotectants, yet their role in lethal Clara cell injury is not clear. To define the pattern of Hsp expression in acute lethal Clara cell injury, mice were treated with the Clara cell-specific toxicant naphthalene (NA), and patterns of expression compared to electrophilic protein adduction and previously established organellar degradation and gluathione (GSH) depletion. In sites of lethal injury (distal bronchiole), prior to organellar degradation (1 h post-NA), protein adduction is detectable and ubiquitin, Hsp 25, Hsp 72, and heme-oxygenase 1 (HO-1) are increased. Maximal Hsp expression, protein adduction, and GSH depletion occur simultaneous (by 2-3 h) with early organelle disruption. Hsp expression is higher later (6-24 h), only in exfoliating cells. In airway sites (proximal bronchiole) with nonlethal Clara cell injury elevation of Hsp 25, 72, and HO-1 expression follows significant GSH depletion (greater than 50% 2 h post-NA). This data build upon our previous studies and we conclude that (1) in lethal (terminal bronchiole) and nonlethal (proximal bronchiole) Clara cell injury, Hsp induction is associated with the loss of GSH and increased protein adduction, and (2) in these same sites, organelle disruption is not a prerequisite for Hsp induction.

Original languageEnglish (US)
Pages (from-to)107-118
Number of pages12
JournalToxicology and Applied Pharmacology
Volume192
Issue number2
DOIs
StatePublished - Oct 15 2003

Keywords

  • Clara cell
  • Heat shock protein
  • Naphthalene
  • Protein adduct

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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