Pseudomonas aeruginosa potentiates the lethal effect of intestinal ischemia-reperfusion injury

The role of in vivo virulence activation

David Fink, Kathleen Romanowski, Vesta Valuckaite, Trissa Babrowski, Moses Kim, Jeffrey B. Matthews, Donald Liu, Olga Zaborina, John C. Alverdy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Experimental models of intestinal ischemia-reperfusion (IIR) injury are invariably performed in mice harboring their normal commensal flora, even though multiple IIR events occur in humans during prolonged intensive care confinement when they are colonized by a highly pathogenic hospital flora. The aims of this study were to determine whether the presence of the human pathogen Pseudomonas aeruginosa in the distal intestine potentiates the lethality of mice exposed to IIR and to determine what role any in vivo virulence activation plays in the observed mortality. Methods: Seven- to 9-week-old C57/BL6 mice were exposed to 15 minutes of superior mesenteric artery occlusion (SMAO) followed by direct intestinal inoculation of 1.0 × 10 colony-forming unit of P. aeruginosa PAO1 into the ileum and observed for mortality. Reiterative studies were performed in separate groups of mice to evaluate both the migration/dissemination pattern and in vivo virulence activation of intestinally inoculated strains using live photon camera imaging of both a constitutive bioluminescent P. aeruginosa PAO1 derivative XEN41 and an inducible reporter derivative of PAO1, the PAO1/lecA:luxCDABE that conditionally expresses the quorum sensing-dependent epithelial disrupting virulence protein PA 1 Lectin (PA-IL). Results: Mice exposed to 15 minutes of SMAO and reperfusion with intestinal inoculation of P. aeruginosa had a significantly increased mortality rate (p < 0.001) of 100% compared with <10% for sham-operated mice intestinally inoculated with P. aeruginosa without SMAO and IIR alone (<50%). Migration/dissemination patterns of P. aeruginosa in mice subjected to IIR demonstrated proximal migration of distally injected strains and translocation to mesenteric lymph nodes, liver, spleen, lung, and kidney. A key role for in vivo virulence expression of the barrier disrupting adhesin PA-IL during IIR was established since its expression was enhanced during IR and mutant strains lacking PA-IL displayed attenuated mortality. CONCLUSIONS: The presence of intestinal P. aeruginosa potentiates the lethal effect of IIR in mice in part due to in vivo virulence activation of its epithelial barrier disrupting protein PA-IL.

Original languageEnglish (US)
Pages (from-to)1575-1582
Number of pages8
JournalJournal of Trauma - Injury, Infection and Critical Care
Volume71
Issue number6
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Reperfusion Injury
Pseudomonas aeruginosa
Virulence
Reperfusion
Ischemia
Superior Mesenteric Artery
Mortality
Quorum Sensing
Critical Care
Photons
Ileum
Lectins
Intestines
Proteins
Theoretical Models
Stem Cells
Spleen
Lymph Nodes
Kidney
Lung

Keywords

  • Intestinal ischemia-reperfusion
  • Pseudomonas aeruginosa
  • Small bowel bacterial overgrowth
  • Virulence activation
  • Virulence transcription regulation

ASJC Scopus subject areas

  • Surgery
  • Critical Care and Intensive Care Medicine

Cite this

Pseudomonas aeruginosa potentiates the lethal effect of intestinal ischemia-reperfusion injury : The role of in vivo virulence activation. / Fink, David; Romanowski, Kathleen; Valuckaite, Vesta; Babrowski, Trissa; Kim, Moses; Matthews, Jeffrey B.; Liu, Donald; Zaborina, Olga; Alverdy, John C.

In: Journal of Trauma - Injury, Infection and Critical Care, Vol. 71, No. 6, 01.12.2011, p. 1575-1582.

Research output: Contribution to journalArticle

Fink, David ; Romanowski, Kathleen ; Valuckaite, Vesta ; Babrowski, Trissa ; Kim, Moses ; Matthews, Jeffrey B. ; Liu, Donald ; Zaborina, Olga ; Alverdy, John C. / Pseudomonas aeruginosa potentiates the lethal effect of intestinal ischemia-reperfusion injury : The role of in vivo virulence activation. In: Journal of Trauma - Injury, Infection and Critical Care. 2011 ; Vol. 71, No. 6. pp. 1575-1582.
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abstract = "Background: Experimental models of intestinal ischemia-reperfusion (IIR) injury are invariably performed in mice harboring their normal commensal flora, even though multiple IIR events occur in humans during prolonged intensive care confinement when they are colonized by a highly pathogenic hospital flora. The aims of this study were to determine whether the presence of the human pathogen Pseudomonas aeruginosa in the distal intestine potentiates the lethality of mice exposed to IIR and to determine what role any in vivo virulence activation plays in the observed mortality. Methods: Seven- to 9-week-old C57/BL6 mice were exposed to 15 minutes of superior mesenteric artery occlusion (SMAO) followed by direct intestinal inoculation of 1.0 × 10 colony-forming unit of P. aeruginosa PAO1 into the ileum and observed for mortality. Reiterative studies were performed in separate groups of mice to evaluate both the migration/dissemination pattern and in vivo virulence activation of intestinally inoculated strains using live photon camera imaging of both a constitutive bioluminescent P. aeruginosa PAO1 derivative XEN41 and an inducible reporter derivative of PAO1, the PAO1/lecA:luxCDABE that conditionally expresses the quorum sensing-dependent epithelial disrupting virulence protein PA 1 Lectin (PA-IL). Results: Mice exposed to 15 minutes of SMAO and reperfusion with intestinal inoculation of P. aeruginosa had a significantly increased mortality rate (p < 0.001) of 100{\%} compared with <10{\%} for sham-operated mice intestinally inoculated with P. aeruginosa without SMAO and IIR alone (<50{\%}). Migration/dissemination patterns of P. aeruginosa in mice subjected to IIR demonstrated proximal migration of distally injected strains and translocation to mesenteric lymph nodes, liver, spleen, lung, and kidney. A key role for in vivo virulence expression of the barrier disrupting adhesin PA-IL during IIR was established since its expression was enhanced during IR and mutant strains lacking PA-IL displayed attenuated mortality. CONCLUSIONS: The presence of intestinal P. aeruginosa potentiates the lethal effect of IIR in mice in part due to in vivo virulence activation of its epithelial barrier disrupting protein PA-IL.",
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AU - Babrowski, Trissa

AU - Kim, Moses

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AU - Zaborina, Olga

AU - Alverdy, John C.

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