Abstract
Fluid transport across cultures of bovine tracheal epithelium was measured with a capacitance probe technique. Baseline fluid absorption (Jv) across bovine cells of 3.2 μl·cm(-2·h-1 was inhibited by ~78% after 1 h of exposure to suspensions of Pseudomonas aeruginosa, with a concomitant decrease in transepithelial potential (TEP) and increase in transepithelial resistance (Rt). Effects of P. aeruginosa were blocked by amiloride, which decreased J(v) by 112% from baseline of 2.35 ± 1.25 μl·cm-2·h-1, increased R(t) by 101% from baseline of 610 ± 257 Ω·cm2, and decreased TEP by 91% from baseline of -55 ± 18.5 mV. Microelectrode studies suggested that effects of P. aeruginosa on amiloride-sensitive Na absorption were due in part to a block of basolateral membrane K channels. In the presence of Cl transport inhibitors [5-nitro-2-(3-phenylpropylamino)-benzoic acid, H2- DIDS, and bumetanide], P. aeruginosa induced a fluid secretion of ~2.5 ± 0.4 μl·cm-2·h-1 and decreased (Rt) without changing TEP. However, these changes were abolished when the transport inhibitors were used in a medium in which Cl was replaced by an impermeant organic anion. Filtrates of P. aeruginosa suspensions had no effect on J(v) TEP, or R(t). Mutants lacking exotoxin A or rhamnolipids or with defective lipopolysaccharide still inhibited fluid absorption and altered bioelectrical properties. By contrast, mutations in the rpoN gene encoding a δ factor of RNA polymerase abolished actions of P. aeruginosa. In vivo, changes in transepithelial salt and water transport induced by P. aeruginosa may alter viscosity and ionic composition of airway secretions so as to foster further bacterial colonization.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 275 |
| Issue number | 5 44-5 |
| State | Published - 1998 |
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Keywords
- Bacteria
- RpoN
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
- Physiology (medical)
Cite this
Pseudomonas aeruginosa induces changes in fluid transport across airway surface epithelia. / Evans, D. J.; Matsumoto, P. S.; Widdicombe, Jonathan; Li-Yun, C.; Maminishkis, A. A.; Miller, S. S.
In: American Journal of Physiology - Cell Physiology, Vol. 275, No. 5 44-5, 1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pseudomonas aeruginosa induces changes in fluid transport across airway surface epithelia
AU - Evans, D. J.
AU - Matsumoto, P. S.
AU - Widdicombe, Jonathan
AU - Li-Yun, C.
AU - Maminishkis, A. A.
AU - Miller, S. S.
PY - 1998
Y1 - 1998
N2 - Fluid transport across cultures of bovine tracheal epithelium was measured with a capacitance probe technique. Baseline fluid absorption (Jv) across bovine cells of 3.2 μl·cm(-2·h-1 was inhibited by ~78% after 1 h of exposure to suspensions of Pseudomonas aeruginosa, with a concomitant decrease in transepithelial potential (TEP) and increase in transepithelial resistance (Rt). Effects of P. aeruginosa were blocked by amiloride, which decreased J(v) by 112% from baseline of 2.35 ± 1.25 μl·cm-2·h-1, increased R(t) by 101% from baseline of 610 ± 257 Ω·cm2, and decreased TEP by 91% from baseline of -55 ± 18.5 mV. Microelectrode studies suggested that effects of P. aeruginosa on amiloride-sensitive Na absorption were due in part to a block of basolateral membrane K channels. In the presence of Cl transport inhibitors [5-nitro-2-(3-phenylpropylamino)-benzoic acid, H2- DIDS, and bumetanide], P. aeruginosa induced a fluid secretion of ~2.5 ± 0.4 μl·cm-2·h-1 and decreased (Rt) without changing TEP. However, these changes were abolished when the transport inhibitors were used in a medium in which Cl was replaced by an impermeant organic anion. Filtrates of P. aeruginosa suspensions had no effect on J(v) TEP, or R(t). Mutants lacking exotoxin A or rhamnolipids or with defective lipopolysaccharide still inhibited fluid absorption and altered bioelectrical properties. By contrast, mutations in the rpoN gene encoding a δ factor of RNA polymerase abolished actions of P. aeruginosa. In vivo, changes in transepithelial salt and water transport induced by P. aeruginosa may alter viscosity and ionic composition of airway secretions so as to foster further bacterial colonization.
AB - Fluid transport across cultures of bovine tracheal epithelium was measured with a capacitance probe technique. Baseline fluid absorption (Jv) across bovine cells of 3.2 μl·cm(-2·h-1 was inhibited by ~78% after 1 h of exposure to suspensions of Pseudomonas aeruginosa, with a concomitant decrease in transepithelial potential (TEP) and increase in transepithelial resistance (Rt). Effects of P. aeruginosa were blocked by amiloride, which decreased J(v) by 112% from baseline of 2.35 ± 1.25 μl·cm-2·h-1, increased R(t) by 101% from baseline of 610 ± 257 Ω·cm2, and decreased TEP by 91% from baseline of -55 ± 18.5 mV. Microelectrode studies suggested that effects of P. aeruginosa on amiloride-sensitive Na absorption were due in part to a block of basolateral membrane K channels. In the presence of Cl transport inhibitors [5-nitro-2-(3-phenylpropylamino)-benzoic acid, H2- DIDS, and bumetanide], P. aeruginosa induced a fluid secretion of ~2.5 ± 0.4 μl·cm-2·h-1 and decreased (Rt) without changing TEP. However, these changes were abolished when the transport inhibitors were used in a medium in which Cl was replaced by an impermeant organic anion. Filtrates of P. aeruginosa suspensions had no effect on J(v) TEP, or R(t). Mutants lacking exotoxin A or rhamnolipids or with defective lipopolysaccharide still inhibited fluid absorption and altered bioelectrical properties. By contrast, mutations in the rpoN gene encoding a δ factor of RNA polymerase abolished actions of P. aeruginosa. In vivo, changes in transepithelial salt and water transport induced by P. aeruginosa may alter viscosity and ionic composition of airway secretions so as to foster further bacterial colonization.
KW - Bacteria
KW - RpoN
UR - http://www.scopus.com/inward/record.url?scp=0031731370&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031731370&partnerID=8YFLogxK
M3 - Article
C2 - 9814977
AN - SCOPUS:0031731370
VL - 275
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 5 44-5
ER -