Pseudocleavage Is Dispensable for Polarity and Development in C. elegans Embryos

Lesilee S. Rose, Melissa L. Lamb, Steven N. Hird, Kenneth J. Kemphues

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The first cleavage of the Caenorhabditis elegans embryo is asymmetrical, producing daughters with different cell fates. During the first cell cycle, P granules, cytoplasmic components that are segregated to the germ-line, are localized to the posterior of the embryo. It has been hypothesized that the asymmetrical behavior of the daughters of the first division results from a similar localization of developmental determinants. A process called pseudocleavage also occurs during the first cell cycle: Anterior cortical contractions culminate in a single partial constriction of the embryo called the pseudocleavage furrow. Coincident with pseudocleavage, there is an anteriorly directed flow of cortical cytoplasm and a posteriorly directed flow of internal cytoplasm. Foci of filamentous cortical actin become asymmetrically distributed into an anterior cap. Roles for these various first cell cycle events in cytoplasmic localization and development have been suggested but remain unclear. We have isolated a maternal effect mutation, nop-1 (it142), which abolishes the anterior cortical contractions and the pseudocleavage furrow. In addition, cortical actin foci remain uniformly distributed in most embryos. Despite these defects, cytoplasmic and cortical streaming is present and P granules are localized to the posterior of early embryos. In most embryos from mutant mothers, development proceeds normally and the embryos hatch and grow into fertile adults. We conclude that the pseudocleavage contractions and furrow are dispensable for the development of C. elegans.

Original languageEnglish (US)
Pages (from-to)479-489
Number of pages11
JournalDevelopmental Biology
Volume168
Issue number2
DOIs
StatePublished - Apr 1995
Externally publishedYes

Fingerprint

Body Patterning
Embryonic Structures
Cell Cycle
Caenorhabditis elegans
Actins
Cytoplasm
Cytoplasmic Streaming
Cytoplasmic Granules
Constriction
Germ Cells
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Pseudocleavage Is Dispensable for Polarity and Development in C. elegans Embryos. / Rose, Lesilee S.; Lamb, Melissa L.; Hird, Steven N.; Kemphues, Kenneth J.

In: Developmental Biology, Vol. 168, No. 2, 04.1995, p. 479-489.

Research output: Contribution to journalArticle

Rose, Lesilee S. ; Lamb, Melissa L. ; Hird, Steven N. ; Kemphues, Kenneth J. / Pseudocleavage Is Dispensable for Polarity and Development in C. elegans Embryos. In: Developmental Biology. 1995 ; Vol. 168, No. 2. pp. 479-489.
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