Abstract
The present study aims to define the role of postsynaptic density (PSD)-95 in the regulation of dopamine (DA) receptor function. We found that PSD-95 physically associates with either D1 or D2 DA receptors in co-transfected HEK-293 cells. Stimulation of DA receptors altered the association between D1 receptor and PSD-95 in a time-dependent manner. Functional assays indicated that PSD-95 co-expression did not affect D1 receptor-stimulated cAMP production, Gs-protein activation or receptor desensitization. However, PSD-95 accelerated the recovery of internalized membrane receptors by promoting receptor recycling, thus resulting in enhanced resensitization of internalized D1 receptors. Our results provide a novel mechanism for regulating DA receptor recycling that may play an important role in postsynaptic DA functional modulation and synaptic neuroplasticity.
Original language | English (US) |
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Pages (from-to) | 612-624 |
Number of pages | 13 |
Journal | Cell Research |
Volume | 19 |
Issue number | 5 |
DOIs | |
State | Published - May 2009 |
Keywords
- Desensitization
- Dopamine receptor
- Gs-protein activation
- PSD-95
- Recycling
- Resensitization
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology