PSD-95 regulates D1 dopamine receptor resensitization,but not receptor-mediated Gs-protein activation

Peihua Sun, Jingru Wang, Weihua Gu, Wei Cheng, Guo Zhang Jin, Eitan Friedman, Jie Zheng, Xuechu Zhen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The present study aims to define the role of postsynaptic density (PSD)-95 in the regulation of dopamine (DA) receptor function. We found that PSD-95 physically associates with either D1 or D2 DA receptors in co-transfected HEK-293 cells. Stimulation of DA receptors altered the association between D1 receptor and PSD-95 in a time-dependent manner. Functional assays indicated that PSD-95 co-expression did not affect D1 receptor-stimulated cAMP production, Gs-protein activation or receptor desensitization. However, PSD-95 accelerated the recovery of internalized membrane receptors by promoting receptor recycling, thus resulting in enhanced resensitization of internalized D1 receptors. Our results provide a novel mechanism for regulating DA receptor recycling that may play an important role in postsynaptic DA functional modulation and synaptic neuroplasticity.

Original languageEnglish (US)
Pages (from-to)612-624
Number of pages13
JournalCell Research
Volume19
Issue number5
DOIs
StatePublished - May 2009

Fingerprint

Post-Synaptic Density
Dopamine D1 Receptors
Dopamine Receptors
Proteins
Cyclic AMP Receptors
Neuronal Plasticity
Dopamine D2 Receptors
HEK293 Cells
Dopamine
Membranes

Keywords

  • Desensitization
  • Dopamine receptor
  • Gs-protein activation
  • PSD-95
  • Recycling
  • Resensitization

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Sun, P., Wang, J., Gu, W., Cheng, W., Jin, G. Z., Friedman, E., ... Zhen, X. (2009). PSD-95 regulates D1 dopamine receptor resensitization,but not receptor-mediated Gs-protein activation. Cell Research, 19(5), 612-624. https://doi.org/10.1038/cr.2009.30

PSD-95 regulates D1 dopamine receptor resensitization,but not receptor-mediated Gs-protein activation. / Sun, Peihua; Wang, Jingru; Gu, Weihua; Cheng, Wei; Jin, Guo Zhang; Friedman, Eitan; Zheng, Jie; Zhen, Xuechu.

In: Cell Research, Vol. 19, No. 5, 05.2009, p. 612-624.

Research output: Contribution to journalArticle

Sun, P, Wang, J, Gu, W, Cheng, W, Jin, GZ, Friedman, E, Zheng, J & Zhen, X 2009, 'PSD-95 regulates D1 dopamine receptor resensitization,but not receptor-mediated Gs-protein activation', Cell Research, vol. 19, no. 5, pp. 612-624. https://doi.org/10.1038/cr.2009.30
Sun, Peihua ; Wang, Jingru ; Gu, Weihua ; Cheng, Wei ; Jin, Guo Zhang ; Friedman, Eitan ; Zheng, Jie ; Zhen, Xuechu. / PSD-95 regulates D1 dopamine receptor resensitization,but not receptor-mediated Gs-protein activation. In: Cell Research. 2009 ; Vol. 19, No. 5. pp. 612-624.
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