Proximal clustering between BK and Cav1.3 channels promotes functional coupling and BK channel activation at low voltage

Oscar Vivas, Claudia M. Moreno, Luis Fernando Santana, Bertil Hille

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

CaV-channel dependent activation of BK channels is critical for feedback control of both calcium influx and cell excitability. Here we addressed the functional and spatial interaction between BK and CaV1.3 channels, unique CaV1 channels that activate at low voltages. We found that when BK and CaV1.3 channels were co-expressed in the same cell, BK channels started activating near 50 mV, ~30 mV more negative than for activation of co-expressed BK and high-voltage activated CaV2.2 channels. In addition, single-molecule localization microscopy revealed striking clusters of CaV1.3 channels surrounding clusters of BK channels and forming a multi-channel complex both in a heterologous system and in rat hippocampal and sympathetic neurons. We propose that this spatial arrangement allows tight tracking between local BK channel activation and the gating of CaV1.3 channels at quite negative membrane potentials, facilitating the regulation of neuronal excitability at voltages close to the threshold to fire action potentials.

Original languageEnglish (US)
Article numbere28029
JournaleLife
Volume6
DOIs
StatePublished - Jun 30 2017

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Large-Conductance Calcium-Activated Potassium Channels
Cluster Analysis
Chemical activation
Electric potential
Neurons
Feedback control
Rats
Microscopic examination
Fires
Calcium
Membranes
Membrane Potentials
Action Potentials
Molecules

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Proximal clustering between BK and Cav1.3 channels promotes functional coupling and BK channel activation at low voltage. / Vivas, Oscar; Moreno, Claudia M.; Santana, Luis Fernando; Hille, Bertil.

In: eLife, Vol. 6, e28029, 30.06.2017.

Research output: Contribution to journalArticle

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