Abstract
Feline immunodeficiency virus (FIV) is similar to human immunodeficiency virus type 1 virologically and induces a clinical syndrome in cats comparable to human immunodeficiency virus type 1 syndrome in humans. To determine the lymphoid target cells of FIV, populations of CD4 + lymphocytes, CD8 + lymphocytes, and CD21 + lymphocytes (B cells) were enriched to more than 96.5% purity and then analyzed for FIV provirus by semiquantitative DNA amplification. We found FIV provirus in CD4 +, CD8 +, and B lymphocytes. In cats infected for <4 months, proviral burden was greatest in CD4 + cells, followed by B cells and then by CD8 + cells. In cats infected for more than 5 years, proviral burden was greatest in B cells, followed by CD4 + cells and then by CD8 + cells. The total proviral burden was >1 log 10 higher in acutely infected cats than in chronically infected cats, primarily because of a higher level of CD4 + infection in the acutely infected cats. A comparison of proviral loads in mesenteric lymph node and peripheral blood mononuclear cells in acutely or chronically infected cats revealed no significant difference. A kinetics study of FIV infection demonstrated that all lymphocyte subpopulations were infected by 4 weeks postinoculation. Virus was isolated from CD4 +, CD8 +, and B cells in vitro, and reverse transcriptase PER demonstrated that all subsets contained viral RNA in vivo and therefore are productive reservoirs for FIV.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5165-5169 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 70 |
| Issue number | 8 |
| State | Published - Aug 1996 |
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ASJC Scopus subject areas
- Immunology
Cite this
Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node. / Dean, Gregg A.; Reubel, Gehard H.; Moore, Peter F; Pedersen, Niels C.
In: Journal of Virology, Vol. 70, No. 8, 08.1996, p. 5165-5169.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proviral burden and infection kinetics of feline immunodeficiency virus in lymphocyte subsets of blood and lymph node
AU - Dean, Gregg A.
AU - Reubel, Gehard H.
AU - Moore, Peter F
AU - Pedersen, Niels C
PY - 1996/8
Y1 - 1996/8
N2 - Feline immunodeficiency virus (FIV) is similar to human immunodeficiency virus type 1 virologically and induces a clinical syndrome in cats comparable to human immunodeficiency virus type 1 syndrome in humans. To determine the lymphoid target cells of FIV, populations of CD4 + lymphocytes, CD8 + lymphocytes, and CD21 + lymphocytes (B cells) were enriched to more than 96.5% purity and then analyzed for FIV provirus by semiquantitative DNA amplification. We found FIV provirus in CD4 +, CD8 +, and B lymphocytes. In cats infected for <4 months, proviral burden was greatest in CD4 + cells, followed by B cells and then by CD8 + cells. In cats infected for more than 5 years, proviral burden was greatest in B cells, followed by CD4 + cells and then by CD8 + cells. The total proviral burden was >1 log 10 higher in acutely infected cats than in chronically infected cats, primarily because of a higher level of CD4 + infection in the acutely infected cats. A comparison of proviral loads in mesenteric lymph node and peripheral blood mononuclear cells in acutely or chronically infected cats revealed no significant difference. A kinetics study of FIV infection demonstrated that all lymphocyte subpopulations were infected by 4 weeks postinoculation. Virus was isolated from CD4 +, CD8 +, and B cells in vitro, and reverse transcriptase PER demonstrated that all subsets contained viral RNA in vivo and therefore are productive reservoirs for FIV.
AB - Feline immunodeficiency virus (FIV) is similar to human immunodeficiency virus type 1 virologically and induces a clinical syndrome in cats comparable to human immunodeficiency virus type 1 syndrome in humans. To determine the lymphoid target cells of FIV, populations of CD4 + lymphocytes, CD8 + lymphocytes, and CD21 + lymphocytes (B cells) were enriched to more than 96.5% purity and then analyzed for FIV provirus by semiquantitative DNA amplification. We found FIV provirus in CD4 +, CD8 +, and B lymphocytes. In cats infected for <4 months, proviral burden was greatest in CD4 + cells, followed by B cells and then by CD8 + cells. In cats infected for more than 5 years, proviral burden was greatest in B cells, followed by CD4 + cells and then by CD8 + cells. The total proviral burden was >1 log 10 higher in acutely infected cats than in chronically infected cats, primarily because of a higher level of CD4 + infection in the acutely infected cats. A comparison of proviral loads in mesenteric lymph node and peripheral blood mononuclear cells in acutely or chronically infected cats revealed no significant difference. A kinetics study of FIV infection demonstrated that all lymphocyte subpopulations were infected by 4 weeks postinoculation. Virus was isolated from CD4 +, CD8 +, and B cells in vitro, and reverse transcriptase PER demonstrated that all subsets contained viral RNA in vivo and therefore are productive reservoirs for FIV.
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M3 - Article
VL - 70
SP - 5165
EP - 5169
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 8
ER -