Protrusive push versus enveloping embrace: Computational model of phagocytosis predicts key regulatory role of cytoskeletal membrane anchors

Marc Herant, Cheng Yuk Lee, Micah Dembo, Volkmar Heinrich

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Encounters between human neutrophils and zymosan elicit an initially protrusive cell response that is distinct from the thin lamella embracing antibody-coated targets. Recent experiments have led us to hypothesize that this behavior has its mechanistic roots in the modulation of interactions between membrane and cytoskeleton. To test and refine this hypothesis, we confront our experimental results with predictions of a computer model of leukocyte mechanical behavior, and establish the minimum set of mechanistic variations of this computational framework that reproduces the differences between zymosan and antibody phagocytosis. We confirm that the structural linkages between the cytoskeleton and the membrane patch adherent to a target form the "switchboard" that controls the target specificity of a neutrophil's mechanical response. These linkages are presumably actin-binding protein complexes associating with the cytoplasmic domains of cell-surface receptors that are engaged in adhesion to zymosan and Fc-domains.

Original languageEnglish (US)
Article numbere1001068
JournalPLoS Computational Biology
Volume7
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

zymosan
Zymosan
Anchors
phagocytosis
anchor
Phagocytosis
Antibodies
Computational Model
antibody
Neutrophils
Cytoskeleton
Membrane
membrane
cytoskeleton
Membranes
Antibody
Predict
Linkage
microfilament proteins
Target

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Ecology
  • Molecular Biology
  • Genetics
  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Computational Theory and Mathematics

Cite this

Protrusive push versus enveloping embrace : Computational model of phagocytosis predicts key regulatory role of cytoskeletal membrane anchors. / Herant, Marc; Lee, Cheng Yuk; Dembo, Micah; Heinrich, Volkmar.

In: PLoS Computational Biology, Vol. 7, No. 1, e1001068, 01.2011.

Research output: Contribution to journalArticle

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