Protocol to record and quantify the intracellular pH in contracting cardiomyocytes

Yankun Lyu, Valeriy Timofeyev, James Overton, Phung N. Thai, Ebenezer N Yamoah, Nipavan Chiamvimonvat, Xiaodong Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Intracellular pH (pHi) plays critical roles in the regulation of cardiac function. Methods and techniques for cardiac pHi measurement have continued to evolve since early 1960s. Fluorescent microscopy is the most recently developed technique with several advantages over other techniques including higher spatial and temporal resolutions, and feasibility for contracting cell measurement. Here, we describe detailed methods for mouse cardiomyocyte isolation, and simultaneous measurement and quantification of pHi and sarcomere length in contracting cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Lyu et al. (2022).

Original languageEnglish (US)
Article number101301
JournalSTAR Protocols
Volume3
Issue number2
DOIs
StatePublished - Jun 17 2022

Keywords

  • Cell isolation
  • Cell-based Assays
  • Metabolism
  • Microscopy
  • Single Cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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