Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Xiao Dong Zhang; Maria Cristina Perez Flores; Valeriy Timofeyev; Ebenezer N Yamoah; Nipavan Chiamvimonvat

doi:10.1016/j.xpro.2021.100891

Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Xiao Dong Zhang, Maria Cristina Perez Flores, Valeriy Timofeyev, Ebenezer N Yamoah, Nipavan Chiamvimonvat

Cardiology (Davis)

Research output: Contribution to journal › Article › peer-review

Abstract

Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

Original language	English (US)
Article number	100891
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100891
State	Published - Dec 17 2021

Keywords

Biophysics
Cell Biology
Cell isolation
Health Sciences
Single Cell

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)

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10.1016/j.xpro.2021.100891

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title = "Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes",

abstract = "Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).",

keywords = "Biophysics, Cell Biology, Cell isolation, Health Sciences, Single Cell",

author = "Zhang, {Xiao Dong} and Flores, {Maria Cristina Perez} and Valeriy Timofeyev and Yamoah, {Ebenezer N} and Nipavan Chiamvimonvat",

note = "Funding Information: We thank our laboratory members for their constructive comments. The study was supported in part by the American Heart Association Beginning Grant-in-Aid 14BGIA18870087 and NIH R56 HL138392 (X.-D.Z.); NIH R01 DC016099 , NIH R01 DC015252 , NIH R01 DC015135 , and NIH P01 AG051443 (E.N.Y.); NIH R01 HL085727 , NIH R01 HL085844 , NIH R01 HL137228 , and NIH S10 RR033106 (N.C.); and VA Merit Review Grant I01 BX000576 and I01 CX001490 (N.C.). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

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doi = "10.1016/j.xpro.2021.100891",

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AU - Zhang, Xiao Dong

AU - Flores, Maria Cristina Perez

AU - Timofeyev, Valeriy

AU - Yamoah, Ebenezer N

AU - Chiamvimonvat, Nipavan

N1 - Funding Information: We thank our laboratory members for their constructive comments. The study was supported in part by the American Heart Association Beginning Grant-in-Aid 14BGIA18870087 and NIH R56 HL138392 (X.-D.Z.); NIH R01 DC016099 , NIH R01 DC015252 , NIH R01 DC015135 , and NIH P01 AG051443 (E.N.Y.); NIH R01 HL085727 , NIH R01 HL085844 , NIH R01 HL137228 , and NIH S10 RR033106 (N.C.); and VA Merit Review Grant I01 BX000576 and I01 CX001490 (N.C.). Publisher Copyright: © 2021 The Author(s)

PY - 2021/12/17

Y1 - 2021/12/17

N2 - Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

AB - Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

KW - Biophysics

KW - Cell Biology

KW - Cell isolation

KW - Health Sciences

KW - Single Cell

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Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Abstract

Keywords

ASJC Scopus subject areas

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