Protocol to assess two distinct components of the nonlinear capacitance in mouse cardiomyocytes

Xiao Dong Zhang, Maria Cristina Perez Flores, Valeriy Timofeyev, Ebenezer N Yamoah, Nipavan Chiamvimonvat

Research output: Contribution to journalArticlepeer-review

Abstract

Prestin (Slc26a5) is a motor protein previously considered to be expressed exclusively in outer hair cells (OHCs) of the inner ear. However, we recently identified the functional expression of prestin in the heart. Nonlinear capacitance (NLC) measurement in OHCs is used to evaluate the signature function of prestin, which exhibits membrane potential-dependent conformational changes. Here, we describe detailed recording techniques and quantification methods for NLC to evaluate the prestin function in mouse ventricular myocytes. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

Original languageEnglish (US)
Article number100891
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021

Keywords

  • Biophysics
  • Cell Biology
  • Cell isolation
  • Health Sciences
  • Single Cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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