Proteomics in primary biliary cholangitis

You Li, Ruqui Tang, Patrick S Leung, M. Eric Gershwin, Xiong Ma

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Primary biliary cholangitis is a chronic cholestatic liver disease characterized by the presence of serum antimitochondrial antibodies and immune-mediated destruction of the small and medium-sized intrahepatic bile ducts. However, the pathophysiology of primary biliary cholangitis has not yet been completely elucidated. In recent years, proteomics has been comprehensively applied in many research fields, including the pathogenesis, prognosis, and diagnosis of disease. Among multiple methods, isobaric tag for relative and absolute quantitation is a powerful analytic method to characterize complex protein mixtures in combination with liquid chromatography-tandem mass spectrometry. In this chapter, we describe a strategy for using isobaric tag for relative and absolute quantitation to discover those differentially expressed proteins in primary biliary cholangitis. The goal is to identify the differences in protein expression between patients with primary biliary cholangitis and healthy controls for defining biomarkers and elucidating molecular mechanisms underlying disease states.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-173
Number of pages11
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1981
ISSN (Print)1064-3745

Keywords

  • Bioinformatics
  • Differentially expressed proteins
  • Full-scan MS spectra
  • High pH reverse phase liquid chromatography (RHPLC)
  • Isobaric tag for relative and absolute quantitation (iTRAQ)
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
  • Plasma
  • Primary biliary cholangitis
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Li, Y., Tang, R., Leung, P. S., Gershwin, M. E., & Ma, X. (2019). Proteomics in primary biliary cholangitis. In Methods in Molecular Biology (pp. 163-173). (Methods in Molecular Biology; Vol. 1981). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9420-5_11