TY - JOUR
T1 - Proteomic characterization of Yersinia pestis virulence
AU - Chromy, Brett A.
AU - Choi, Megan W.
AU - Murphy, Gloria A.
AU - Gonzales, Arlene D.
AU - Corzett, Chris H.
AU - Chang, Brian C.
AU - Fitch, J. Patrick
AU - McCutchen-Maloney, Sandra L.
PY - 2005/12
Y1 - 2005/12
N2 - The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
AB - The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
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U2 - 10.1128/JB.187.23.8172-8180.2005
DO - 10.1128/JB.187.23.8172-8180.2005
M3 - Article
C2 - 16291690
AN - SCOPUS:17244376424
VL - 187
SP - 8172
EP - 8180
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 23
ER -