Abstract
The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8172-8180 |
| Number of pages | 9 |
| Journal | Journal of Bacteriology |
| Volume | 187 |
| Issue number | 23 |
| DOIs | |
| State | Published - Dec 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Proteomic characterization of Yersinia pestis virulence. / Chromy, Brett A.; Choi, Megan W.; Murphy, Gloria A.; Gonzales, Arlene D.; Corzett, Chris H.; Chang, Brian C.; Fitch, J. Patrick; McCutchen-Maloney, Sandra L.
In: Journal of Bacteriology, Vol. 187, No. 23, 12.2005, p. 8172-8180.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic characterization of Yersinia pestis virulence
AU - Chromy, Brett A.
AU - Choi, Megan W.
AU - Murphy, Gloria A.
AU - Gonzales, Arlene D.
AU - Corzett, Chris H.
AU - Chang, Brian C.
AU - Fitch, J. Patrick
AU - McCutchen-Maloney, Sandra L.
PY - 2005/12
Y1 - 2005/12
N2 - The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
AB - The Yersinia pestis proteome was studied as a function of temperature and calcium by two-dimensional differential gel electrophoresis. Over 4,100 individual protein spots were detected, of which hundreds were differentially expressed. A total of 43 differentially expressed protein spots, representing 24 unique proteins, were identified by mass spectrometry. Differences in expression were observed for several virulence-associated factors, including catalase-peroxidase (KatY), murine toxin (Ymt), plasminogen activator (Pla), and F1 capsule antigen (Caf1), as well as several putative virulence factors and membrane-bound and metabolic proteins. Differentially expressed proteins not previously reported to contribute to virulence are candidates for more detailed mechanistic studies, representing potential new virulence determinants.
UR - http://www.scopus.com/inward/record.url?scp=17244376424&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17244376424&partnerID=8YFLogxK
U2 - 10.1128/JB.187.23.8172-8180.2005
DO - 10.1128/JB.187.23.8172-8180.2005
M3 - Article
C2 - 16291690
AN - SCOPUS:17244376424
VL - 187
SP - 8172
EP - 8180
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 23
ER -