Proteomic characterization of host response to Yersinia pestis and near neighbors

Brett A. Chromy, Julie Perkins, Jenny L. Heidbrink, Arlene D. Gonzales, Gloria A. Murphy, J. Patrick Fitch, Sandra L. McCutchen-Maloney

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Host-pathogen interactions result in protein expression changes within both the host and the pathogen. Here, results from proteomic characterization of host response following exposure to Yersinia pestis, the causative agent of plague, and to two near neighbors, Yersinia pseudotuberculosis and Yersinia enterocolitica, are reported. Human monocyte-like cells were chosen as a model for macrophage immune response to pathogen exposure. Two-dimensional electrophoresis followed by mass spectrometry was used to identify host proteins with differential expression following exposure to these three closely related Yersinia species. This comparative proteomic characterization of host response clearly shows that host protein expression patterns are distinct for the different pathogen exposures, and contributes to further understanding of Y. pestis virulence and host defense mechanisms. This work also lays the foundation for future studies aimed at defining biomarkers for presymptomatic detection of plague.

Original languageEnglish (US)
Pages (from-to)474-479
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number2
DOIs
StatePublished - Jul 23 2004
Externally publishedYes

Keywords

  • Biomarkers
  • Host-pathogen interactions
  • Presymptomatic detection
  • Proteomics
  • Two-dimensional electrophoresis
  • Yersinia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Chromy, B. A., Perkins, J., Heidbrink, J. L., Gonzales, A. D., Murphy, G. A., Fitch, J. P., & McCutchen-Maloney, S. L. (2004). Proteomic characterization of host response to Yersinia pestis and near neighbors. Biochemical and Biophysical Research Communications, 320(2), 474-479. https://doi.org/10.1016/j.bbrc.2004.05.199