Abstract
Host-pathogen interactions result in protein expression changes within both the host and the pathogen. Here, results from proteomic characterization of host response following exposure to Yersinia pestis, the causative agent of plague, and to two near neighbors, Yersinia pseudotuberculosis and Yersinia enterocolitica, are reported. Human monocyte-like cells were chosen as a model for macrophage immune response to pathogen exposure. Two-dimensional electrophoresis followed by mass spectrometry was used to identify host proteins with differential expression following exposure to these three closely related Yersinia species. This comparative proteomic characterization of host response clearly shows that host protein expression patterns are distinct for the different pathogen exposures, and contributes to further understanding of Y. pestis virulence and host defense mechanisms. This work also lays the foundation for future studies aimed at defining biomarkers for presymptomatic detection of plague.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 474-479 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 320 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 23 2004 |
| Externally published | Yes |
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Keywords
- Biomarkers
- Host-pathogen interactions
- Presymptomatic detection
- Proteomics
- Two-dimensional electrophoresis
- Yersinia
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Proteomic characterization of host response to Yersinia pestis and near neighbors. / Chromy, Brett A.; Perkins, Julie; Heidbrink, Jenny L.; Gonzales, Arlene D.; Murphy, Gloria A.; Fitch, J. Patrick; McCutchen-Maloney, Sandra L.
In: Biochemical and Biophysical Research Communications, Vol. 320, No. 2, 23.07.2004, p. 474-479.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteomic characterization of host response to Yersinia pestis and near neighbors
AU - Chromy, Brett A.
AU - Perkins, Julie
AU - Heidbrink, Jenny L.
AU - Gonzales, Arlene D.
AU - Murphy, Gloria A.
AU - Fitch, J. Patrick
AU - McCutchen-Maloney, Sandra L.
PY - 2004/7/23
Y1 - 2004/7/23
N2 - Host-pathogen interactions result in protein expression changes within both the host and the pathogen. Here, results from proteomic characterization of host response following exposure to Yersinia pestis, the causative agent of plague, and to two near neighbors, Yersinia pseudotuberculosis and Yersinia enterocolitica, are reported. Human monocyte-like cells were chosen as a model for macrophage immune response to pathogen exposure. Two-dimensional electrophoresis followed by mass spectrometry was used to identify host proteins with differential expression following exposure to these three closely related Yersinia species. This comparative proteomic characterization of host response clearly shows that host protein expression patterns are distinct for the different pathogen exposures, and contributes to further understanding of Y. pestis virulence and host defense mechanisms. This work also lays the foundation for future studies aimed at defining biomarkers for presymptomatic detection of plague.
AB - Host-pathogen interactions result in protein expression changes within both the host and the pathogen. Here, results from proteomic characterization of host response following exposure to Yersinia pestis, the causative agent of plague, and to two near neighbors, Yersinia pseudotuberculosis and Yersinia enterocolitica, are reported. Human monocyte-like cells were chosen as a model for macrophage immune response to pathogen exposure. Two-dimensional electrophoresis followed by mass spectrometry was used to identify host proteins with differential expression following exposure to these three closely related Yersinia species. This comparative proteomic characterization of host response clearly shows that host protein expression patterns are distinct for the different pathogen exposures, and contributes to further understanding of Y. pestis virulence and host defense mechanisms. This work also lays the foundation for future studies aimed at defining biomarkers for presymptomatic detection of plague.
KW - Biomarkers
KW - Host-pathogen interactions
KW - Presymptomatic detection
KW - Proteomics
KW - Two-dimensional electrophoresis
KW - Yersinia
UR - http://www.scopus.com/inward/record.url?scp=2942720645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942720645&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.05.199
DO - 10.1016/j.bbrc.2004.05.199
M3 - Article
C2 - 15219853
AN - SCOPUS:2942720645
VL - 320
SP - 474
EP - 479
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -