Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model

Fuzheng Guo, Hui Nian, Hao Zhang, Lingyun Huang, Ying Tang, Xueyuan Xiao, Dacheng He

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The 70% (or 2/3) partial hepatectomy (PHx) rat liver model provides an effective medium for study of the transition and regulation of hepatocytes from quiescent to proliferating phase. Although the gene expression pattern has come under intense scrutiny, a differential proteomic study could help to reveal the mechanism of how the process is initiated and regulated. The proteomic changes were analyzed in two groups, 7 h after 70% PHx test group and sham-operation control group, by two-dimensional gel electrophoresis with 907 ± 33 and 910 ± 64 spots on gels, respectively. Twelve down-regulated spots and twenty-six up-regulated spots were recognized using ImageMaster software and were identified by matrix-assisted laser desorption/ionization-mass spectrometry-quadrupole time of flight mass spectrometry and/or tandem mass spectrometry reconfirmation. Some of the differential proteins were associated with stress defense, lipid metabolism, and macromolecular biosynthesis while the others were shown to be involved in regulating transcript factors associated with liver regeneration. A "proteomic model" for liver regeneration was suggested based on our data and related scientific literature to interpret the differential proteome pattern that reflected the transition of cells from quiescent to proliferating state, including but not limited to the rat liver after 70% PHx.

Original languageEnglish (US)
Pages (from-to)3075-3086
Number of pages12
JournalProteomics
Volume6
Issue number10
DOIs
StatePublished - May 1 2006
Externally publishedYes

Fingerprint

Hepatectomy
Liver
Proteomics
Rats
Liver Regeneration
Tandem Mass Spectrometry
Mass spectrometry
Literature
Gels
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Proteome
Lipid Metabolism
Hepatocytes
Biosynthesis
Software
Electrophoresis
Gene expression
Ionization
Gene Expression

Keywords

  • Differential proteome
  • Partial hepatectomy
  • Quiescent phase
  • Rat liver
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model. / Guo, Fuzheng; Nian, Hui; Zhang, Hao; Huang, Lingyun; Tang, Ying; Xiao, Xueyuan; He, Dacheng.

In: Proteomics, Vol. 6, No. 10, 01.05.2006, p. 3075-3086.

Research output: Contribution to journalArticle

Guo, Fuzheng ; Nian, Hui ; Zhang, Hao ; Huang, Lingyun ; Tang, Ying ; Xiao, Xueyuan ; He, Dacheng. / Proteomic analysis of the transition from quiescent to proliferating stages in rat liver hepatectomy model. In: Proteomics. 2006 ; Vol. 6, No. 10. pp. 3075-3086.
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