Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes

Susanne R. Berglund, Alison R. Santana, Dan Li, Robert H. Rice, David M Rocke, Zelanna Goldberg

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Human exposure to arsenic and ionizing radiation (IR) occur environmentally at low levels. While the human health effects of arsenic and IR have been examined separately, there is little information regarding their combined effects at doses approaching environmental levels. Arsenic toxicity may be affected by concurrent IR especially given their known individual carcinogenic actions at higher doses. We found that keratinocytes respondedto either low dose arsenic and/or low dose IR exposure, resulting in differential proteomic expression based on 2-DE, immunoblotting and statistical analysis. Seven proteins were identified that passed a rigorous statistical screen for differential expression in 2-DE and also passed a strict statistical screen for follow-up immunoblotting. These included: a-enolase, epidermal-fatty acid binding protein, heat shock protein 27, histidine triad nucleotide-binding protein 1, lactate dehydrogenase A, protein disulfide isomerase precursor, and S100A9. Four proteins had combined effects that were different than would be expected based on the response to either individual toxicant. These data demonstrate a possible reaction to the combined insult that is substantially different from that of either separate treatment. Several proteins had different responses than what has been seen from high dose exposures, adding to the growing literature suggesting that the cellular responses to low dose exposures are distinct.

Original languageEnglish (US)
Pages (from-to)1925-1938
Number of pages14
JournalProteomics
Volume9
Issue number7
DOIs
StatePublished - Apr 2009

Keywords

  • Arsenic
  • Human
  • Ionizing radiation
  • Keratinocyte
  • Low dose

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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