Proteomic analysis of human nail plate

Robert H. Rice, Yajuan Xia, Rudy J. Alvarado, Brett S. Phinney

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Shotgun proteomic analysis of the human nail plate identified 144 proteins in samples from Causcasian volunteers. The 30 identified proteins solubilized by detergent and reducing agent, 90% of the total nail plate mass, were primarily keratins and keratin associated proteins. Keratins comprised a majority of the detergent-insoluble fraction as well, but numerous cytoplasmic, membrane, and junctional proteins and histones were also identified, indicating broad use by transglutaminases of available proteins as substrates for cross-linking. Two novel membrane proteins were identified, also found in the hair shaft, for which mRNAs were detected only at very low levels by real-time polymerase chain reaction in other tissues. Parallel analyses of nail samples from volunteers from Inner Mongolia, China gave essentially the same protein profiles. Comparison of the profiles of nail plate and hair shaft from the latter volunteers revealed extensive overlap of protein constituents. Analyses of samples from an arsenic-exposed population revealed few proteins whose levels were altered substantially but raised the possibility of detecting sensitive individuals in this way.

Original languageEnglish (US)
Pages (from-to)6752-6758
Number of pages7
JournalJournal of Proteome Research
Volume9
Issue number12
DOIs
StatePublished - Dec 3 2010

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Proteomic analysis of human nail plate'. Together they form a unique fingerprint.

Cite this