Proteomic analysis of human keratinocyte response to 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) exposure

Qin Hu, Robert H. Rice, Qin Qin, Brett S. Phinney, Richard A. Eigenheer, Wenjun Bao, Bin Zhao

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Chronic exposure to 2,3,7,8-tetrachlorodibeno-p-dioxin (TCDD) and related polyhalogenated organic pollutants occurs as a consequence of modern life. Exploring the cellular basis for their action is anticipated to help understand the risk they pose and improve the foundation for their regulation. A basis for the striking change in human keratinocyte colony morphology due to TCDD exposure has been investigated by shotgun proteomics. Concentrating on changes in protein levels among three cell strains has revealed significant decreases in the differentiation markers filaggrin, keratin 1, and keratin 10. EGF treatment in concert with TCDD enhanced the changes in these markers and several other proteins while reducing the levels of certain other proteins. The only protein stimulated by TCDD in all three strains and reversed by EGF in them was vimentin, not previously observed to be in the aryl hydrocarbon receptor response domain. Although TCDD is often proposed to enhance keratinocyte differentiation, proteomic analysis reveals it uncouples the differentiation program and suggests that reduced levels of differentiation marker proteins contribute to the observed excessive stratification it induces.

Original languageEnglish (US)
Pages (from-to)5340-5347
Number of pages8
JournalJournal of Proteome Research
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2013

Keywords

  • cell culture
  • cross-linked envelopes
  • dioxin
  • epidermal growth factor
  • filaggrin
  • keratins
  • polyhalogenated organic pollutants
  • vimentin

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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    Hu, Q., Rice, R. H., Qin, Q., Phinney, B. S., Eigenheer, R. A., Bao, W., & Zhao, B. (2013). Proteomic analysis of human keratinocyte response to 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) exposure. Journal of Proteome Research, 12(11), 5340-5347. https://doi.org/10.1021/pr4006266