Proteomic analysis of hair shaft and nail plate

Robert H. Rice

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The protein components ofliving cells in the hair follicle are amenable to study by standard molecular biological techniques, but identifying those in the hair shaft has been problematic until recently. Most of the protein, primarily keratins and keratin associated proteins, can be extracted under denaturing conditions, but 15-20% is intractable due to transglutaminase-mediated cross-linking. Shotgun proteomics now permits identifying >300 constituents of the isopeptide cross-linked proteome and even certain post-translational modifications. The proteins originate from all the intracellular compartments, indicating that the cross-linking process makes effective use of available resources to produce structures with great mechanical stability. Knowing this proteome provides a foundation for correlating defects in hair shaft structure with protein deficiencies. Such investigations can be extended to mouse models of aberrant pelage hair. Thus, inbred mouse strains can be distinguished by their hair proteomes, raising the possibility of similar variation in the human population. The nail plate is also amenable to this shotgun proteomic approach. Providing discrete and noninvasive sampling of the human proteome, these epidermal appendages could have diagnostic utility for certain disease states.

Original languageEnglish (US)
Pages (from-to)229-236
Number of pages8
JournalJournal of Cosmetic Science
Volume62
Issue number2
StatePublished - Mar 2011

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Nails
Proteome
Proteomics
Hair
Firearms
Keratins
Proteins
Protein Deficiency
Inbred Strains Mice
Transglutaminases
Hair Follicle
Mechanical stability
Cellular Structures
Post Translational Protein Processing
Sampling
Defects
Population

ASJC Scopus subject areas

  • Chemistry(all)
  • Dermatology

Cite this

Proteomic analysis of hair shaft and nail plate. / Rice, Robert H.

In: Journal of Cosmetic Science, Vol. 62, No. 2, 03.2011, p. 229-236.

Research output: Contribution to journalArticle

Rice, Robert H. / Proteomic analysis of hair shaft and nail plate. In: Journal of Cosmetic Science. 2011 ; Vol. 62, No. 2. pp. 229-236.
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