Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans

Jae Han Kim, Hyun Joo An, Daniel Garrido, J. Bruce German, Carlito B Lebrilla, David A. Mills

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable of metabolizing human milk oligosaccharides (HMO). To investigate the bacterial response to different prebiotics, we analyzed both cell wall associated and whole cell proteins in B. infantis. Proteins were identified by LC-MS/MS followed by comparative proteomics to deduce the protein localization within the cell. Enzymes involved in the metabolism of lactose, glucose, galactooligosaccharides, fructooligosaccharides and HMO were constitutively expressed exhibiting less than two-fold change regardless of the sugar used. In contrast, enzymes in N-Acetylglucosamine and sucrose catabolism were induced by HMO and fructans, respectively. Galactose-metabolizing enzymes phosphoglucomutase, UDP-glucose 4-epimerase and UTP glucose-1-P uridylytransferase were expressed constitutively, while galactokinase and galactose-1-phosphate uridylyltransferase, increased their expression three fold when HMO and lactose were used as substrates for cell growth. Cell wall-associated proteomics also revealed ATP-dependent sugar transport systems associated with consumption of different prebiotics. In addition, the expression of 16 glycosyl hydrolases revealed the complete metabolic route for each substrate. Mucin, which possesses O-glycans that are structurally similar to HMO did not induced the expression of transport proteins, hydrolysis or sugar metabolic pathway indicating B. infantis do not utilize these glycoconjugates.

Original languageEnglish (US)
Article numbere57535
JournalPLoS One
Volume8
Issue number2
DOIs
StatePublished - Feb 26 2013

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Bifidobacterium longum subsp. infantis
Prebiotics
Human Milk
prebiotics
breast milk
Oligosaccharides
oligosaccharides
Proteomics
proteomics
Polysaccharides
polysaccharides
Sugars
Lactose
sugars
Cell Wall
UTP-hexose-1-phosphate uridylyltransferase
lactose
galactokinase
UDPglucose 4-Epimerase
Enzymes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans. / Kim, Jae Han; An, Hyun Joo; Garrido, Daniel; German, J. Bruce; Lebrilla, Carlito B; Mills, David A.

In: PLoS One, Vol. 8, No. 2, e57535, 26.02.2013.

Research output: Contribution to journalArticle

Kim, Jae Han ; An, Hyun Joo ; Garrido, Daniel ; German, J. Bruce ; Lebrilla, Carlito B ; Mills, David A. / Proteomic Analysis of Bifidobacterium longum subsp. infantis Reveals the Metabolic Insight on Consumption of Prebiotics and Host Glycans. In: PLoS One. 2013 ; Vol. 8, No. 2.
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