Proteome analysis of human hair shaft: From protein identification to posttranslational modification

Young Jin Lee, Robert H. Rice, Young Moo Lee

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

The human hair proteome was investigated using two-dimensional LC-MS/MS. Among the 343 identified proteins, 70 were detected in high relative abundance, including keratin intermediate filament proteins, largely extractable with denaturants. Over 300 proteins were found to constitute the insoluble complex formed by transglutaminase cross-linking. The intracellular distribution of identified proteins is wide from cytoplasm to nucleus, mitochondria, ribosome, and plasma membrane. These results help rationalize ultrastructural features visible in the mature hair. Keratins and several substrates for transglutaminase were found to be posttranslationally modified by methylation and dimethylation. Evidence for ubiquitination of hair proteins was also obtained.

Original languageEnglish (US)
Pages (from-to)789-800
Number of pages12
JournalMolecular and Cellular Proteomics
Volume5
Issue number5
DOIs
StatePublished - May 2006

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Proteome analysis of human hair shaft: From protein identification to posttranslational modification'. Together they form a unique fingerprint.

  • Cite this