Abstract
The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 398-403 |
| Number of pages | 6 |
| Journal | Journal of Dairy Science |
| Volume | 74 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
Fingerprint
Keywords
- amino acid analysis
- Lactobacillus delbrueckii ssp. bulgaricus
- o-phthaldialdehyde test
- OPA
- proteolysis
- thermolactic cultures
- TNBS
- trinitrobenzene sulfonic acid assay
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology
- Genetics
Cite this
Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis. / Oberg, Craig J.; Weimer, Bart C; Moyes, Lynn V.; Brown, Rodney J.; Richardson, Gary H.
In: Journal of Dairy Science, Vol. 74, No. 2, 1991, p. 398-403.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis
AU - Oberg, Craig J.
AU - Weimer, Bart C
AU - Moyes, Lynn V.
AU - Brown, Rodney J.
AU - Richardson, Gary H.
PY - 1991
Y1 - 1991
N2 - The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.
AB - The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.
KW - amino acid analysis
KW - Lactobacillus delbrueckii ssp. bulgaricus
KW - o-phthaldialdehyde test
KW - OPA
KW - proteolysis
KW - thermolactic cultures
KW - TNBS
KW - trinitrobenzene sulfonic acid assay
UR - http://www.scopus.com/inward/record.url?scp=0000700907&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000700907&partnerID=8YFLogxK
U2 - 10.3168/jds.S0022-0302(91)78181-2
DO - 10.3168/jds.S0022-0302(91)78181-2
M3 - Article
AN - SCOPUS:0000700907
VL - 74
SP - 398
EP - 403
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 2
ER -