Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis

Craig J. Oberg, Bart C Weimer, Lynn V. Moyes, Rodney J. Brown, Gary H. Richardson

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.

Original languageEnglish (US)
Pages (from-to)398-403
Number of pages6
JournalJournal of Dairy Science
Volume74
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Lactobacillus delbrueckii
o-Phthalaldehyde
Amino Acids
amino acids
proteolysis
Proteolysis
testing
Trichloroacetic Acid
trichloroacetic acid
Milk Proteins
Ion Exchange
milk proteins
filtrates
ion exchange
Cluster Analysis
cluster analysis
Growth

Keywords

  • amino acid analysis
  • Lactobacillus delbrueckii ssp. bulgaricus
  • o-phthaldialdehyde test
  • OPA
  • proteolysis
  • thermolactic cultures
  • TNBS
  • trinitrobenzene sulfonic acid assay

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis. / Oberg, Craig J.; Weimer, Bart C; Moyes, Lynn V.; Brown, Rodney J.; Richardson, Gary H.

In: Journal of Dairy Science, Vol. 74, No. 2, 1991, p. 398-403.

Research output: Contribution to journalArticle

Oberg, Craig J. ; Weimer, Bart C ; Moyes, Lynn V. ; Brown, Rodney J. ; Richardson, Gary H. / Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis. In: Journal of Dairy Science. 1991 ; Vol. 74, No. 2. pp. 398-403.
@article{783f016d9a1b4e5f9e218d9dcc128b81,
title = "Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis",
abstract = "The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10{\%} NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10{\%} NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.",
keywords = "amino acid analysis, Lactobacillus delbrueckii ssp. bulgaricus, o-phthaldialdehyde test, OPA, proteolysis, thermolactic cultures, TNBS, trinitrobenzene sulfonic acid assay",
author = "Oberg, {Craig J.} and Weimer, {Bart C} and Moyes, {Lynn V.} and Brown, {Rodney J.} and Richardson, {Gary H.}",
year = "1991",
doi = "10.3168/jds.S0022-0302(91)78181-2",
language = "English (US)",
volume = "74",
pages = "398--403",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Proteolytic Characterization of Lactobacillus delbrueckii ssp. bulgaricus Strains by the o-Phthaldialdehyde Test and Amino Acid Analysis

AU - Oberg, Craig J.

AU - Weimer, Bart C

AU - Moyes, Lynn V.

AU - Brown, Rodney J.

AU - Richardson, Gary H.

PY - 1991

Y1 - 1991

N2 - The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.

AB - The o-phthaldialdehyde test and amino acid analysis were used to characterize proteolysis of milk proteins during growth of Lactobacillus delbrueckii ssp. bulgaricus. Thirty-four strains were incubated in sterile 10% NDM for 12 h. Extent of proteolysis as estimated by the o-phthaldialdehyde test revealed a large variance in total proteolysis. For seven strains, trichloroacetic acid filtrates of inoculated 10% NDM were analyzed by classical ion-exchange amino acid analysis. Each strain had a distinct pattern of individual amino acid concentrations. Amino acid profiles provided information about the proteolytic activity of these strains that was not available from the o-phthaldialdehyde test. Cluster analysis, based on amino acid profiles of each strain, differentiated the seven strains beyond what was possible by visual comparison of the amino acid analysis results.

KW - amino acid analysis

KW - Lactobacillus delbrueckii ssp. bulgaricus

KW - o-phthaldialdehyde test

KW - OPA

KW - proteolysis

KW - thermolactic cultures

KW - TNBS

KW - trinitrobenzene sulfonic acid assay

UR - http://www.scopus.com/inward/record.url?scp=0000700907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000700907&partnerID=8YFLogxK

U2 - 10.3168/jds.S0022-0302(91)78181-2

DO - 10.3168/jds.S0022-0302(91)78181-2

M3 - Article

AN - SCOPUS:0000700907

VL - 74

SP - 398

EP - 403

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 2

ER -