Proteolysis of a negative regulator of innate immunity is dependent on resistance genes in tomato and nicotiana benthamiana and induced by multiple bacterial effectors

Yao Luo, Katherine S. Caldwell, Tadeusz Wroblewski, Michael E. Wright, Richard W Michelmore

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

RPM1 -interacting protein 4 (RIN4), a negative regulator of the basal defense response in plants, is targeted by multiple bacterial virulence effectors. We show that RIN4 degradation is induced by the effector AvrPto from Pseudomonas syringae and that this degradation in Solanaceous plants is dependent on the resistance protein, Pto, a protein kinase, and Prf, a nucleotide binding site-leucine-rich repeat protein. Our data demonstrate overlap between two of the best-characterized pathways for recognition of pathogen virulence effectors in plants. RIN4 interacts with multiple plant signaling components and bacterial effectors in yeast and in planta. AvrPto induces an endogenous proteolytic activity in both tomato [Solanum lycopersicum) and Nicotiana benthamiana that degrades RIN4 and requires the consensus site cleaved by the protease effector AvrRpt2. The interaction between AvrPto and Pto, but not the kinase activity of Pto, is required for proteolysis of RIN4. Analysis of many of the effectors comprising the secretome of P. syringae pv tomato DC3000 led to the identification of two additional sequence-unrelated effectors that can also induce degradation of RIN4. Therefore, multiple bacterial effectors besides AvrRpt2 elicit proteolysis of RIN4 in planta.

Original languageEnglish (US)
Pages (from-to)2458-2472
Number of pages15
JournalPlant Cell
Volume21
Issue number8
DOIs
StatePublished - Aug 2009

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Nicotiana benthamiana
Lycopersicon esculentum
Innate Immunity
proteolysis
Proteolysis
Tobacco
tomatoes
Genes
Proteins
genes
proteins
Pseudomonas syringae
Virulence
Plantae
degradation
Plant Structures
virulence
innate immunity
Protein Kinases
Solanum lycopersicum

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Proteolysis of a negative regulator of innate immunity is dependent on resistance genes in tomato and nicotiana benthamiana and induced by multiple bacterial effectors. / Luo, Yao; Caldwell, Katherine S.; Wroblewski, Tadeusz; Wright, Michael E.; Michelmore, Richard W.

In: Plant Cell, Vol. 21, No. 8, 08.2009, p. 2458-2472.

Research output: Contribution to journalArticle

Luo, Yao ; Caldwell, Katherine S. ; Wroblewski, Tadeusz ; Wright, Michael E. ; Michelmore, Richard W. / Proteolysis of a negative regulator of innate immunity is dependent on resistance genes in tomato and nicotiana benthamiana and induced by multiple bacterial effectors. In: Plant Cell. 2009 ; Vol. 21, No. 8. pp. 2458-2472.
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