Abstract
The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 431-438 |
| Number of pages | 8 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 302 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 1 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures. / Schafer, S. J.; Luyten, F. P.; Yanagishita, M.; Reddi, A Hari.
In: Archives of Biochemistry and Biophysics, Vol. 302, No. 2, 01.05.1993, p. 431-438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures
AU - Schafer, S. J.
AU - Luyten, F. P.
AU - Yanagishita, M.
AU - Reddi, A Hari
PY - 1993/5/1
Y1 - 1993/5/1
N2 - The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.
AB - The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027185866&partnerID=8YFLogxK
U2 - 10.1006/abbi.1993.1236
DO - 10.1006/abbi.1993.1236
M3 - Article
C2 - 8489248
AN - SCOPUS:0027185866
VL - 302
SP - 431
EP - 438
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 2
ER -