Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures

S. J. Schafer, F. P. Luyten, M. Yanagishita, A Hari Reddi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume302
Issue number2
DOIs
StatePublished - May 1 1993
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor
Cartilage
Articular Cartilage
Proteoglycans
Metabolism
Protein Isoforms
Animals
Chondroitin ABC Lyase
Glycosaminoglycans
Homeostasis
Biosynthesis
DNA
Monomers
Serum
Tissue
platelet-derived growth factor BB
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures. / Schafer, S. J.; Luyten, F. P.; Yanagishita, M.; Reddi, A Hari.

In: Archives of Biochemistry and Biophysics, Vol. 302, No. 2, 01.05.1993, p. 431-438.

Research output: Contribution to journalArticle

@article{d49d3c56096d4010a7e39a16bd166bd1,
title = "Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures",
abstract = "The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.",
author = "Schafer, {S. J.} and Luyten, {F. P.} and M. Yanagishita and Reddi, {A Hari}",
year = "1993",
month = "5",
day = "1",
doi = "10.1006/abbi.1993.1236",
language = "English (US)",
volume = "302",
pages = "431--438",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Proteoglycan Metabolism Is Age Related and Modulated by Isoforms of Platelet-Derived Growth Factor in Bovine Articular Cartilage Explant Cultures

AU - Schafer, S. J.

AU - Luyten, F. P.

AU - Yanagishita, M.

AU - Reddi, A Hari

PY - 1993/5/1

Y1 - 1993/5/1

N2 - The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.

AB - The influence of recombinant human platelet-derived growth factor (PDGF) on proteoglycan metabolism was examined in bovine articular cartilage explants under serum-free conditions. All three isoforms of PDGF (AB, AA. and BB) dose-dependently increased proteoglycan biosynthesis and decreased the rate of proteoglycan catabolism in cartilage explants obtained from young (1-6 weeks), adolescent (2-6 months), and adult animals (2-3 years). The biosynthetic stimulative effect of PDGFAA was significantly lower than that of PDGF-AB only in adult tissues. The decrease in catabolic rate was more pronounced in younger animals. For culture periods of up to 12 days, the three PDGF isoforms did not have an effect on DNA synthesis. The molecular size of both the proteoglycan monomers and the glycosaminoglycan chains and the chondroitinase ABC sensitive pool decreased with age of the animal, but were not altered by PDGF-AB treatment. These data show that the three recombinant human PDGF isoforms contributed to matrix homeostasis in the articular cartilage explant system, with no apparent mitogenic effect.

UR - http://www.scopus.com/inward/record.url?scp=0027185866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027185866&partnerID=8YFLogxK

U2 - 10.1006/abbi.1993.1236

DO - 10.1006/abbi.1993.1236

M3 - Article

C2 - 8489248

AN - SCOPUS:0027185866

VL - 302

SP - 431

EP - 438

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -