Proteins specified by avian erythroblastosis virus: Coding region localization and identification of a previously undetected erb-B polypeptide

M. L. Privalsky, J. M. Bishop

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Avian erythroblastosis virus (AEV) induces erythroblastosis and sarcomas in chickens. Two domains within the viral genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. By use of hybridization-arrested translations and hybridization-selections of mRNA, we have mapped on the viral genome the polypeptides specified by the erb domains. The results of hybridization-arrest with DNA representing the spliced 5' leader region of the AEV mRNA suggested that the authentic product of the erb-B domain was a 61,000 molecular weight protein, not a 41,000 molecular weight polypeptide previously identified.

Original languageEnglish (US)
Pages (from-to)3958-3962
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number13
StatePublished - 1982

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Alpharetrovirus
Viral Genome
Peptides
Spliced Leader RNA
Molecular Weight
Messenger RNA
Proteins
Sarcoma
Chickens
Carcinogenesis
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AB - Avian erythroblastosis virus (AEV) induces erythroblastosis and sarcomas in chickens. Two domains within the viral genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. By use of hybridization-arrested translations and hybridization-selections of mRNA, we have mapped on the viral genome the polypeptides specified by the erb domains. The results of hybridization-arrest with DNA representing the spliced 5' leader region of the AEV mRNA suggested that the authentic product of the erb-B domain was a 61,000 molecular weight protein, not a 41,000 molecular weight polypeptide previously identified.

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