Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines

Jyh Shi Lin, Chi Wei Lu, Chang Jen Huang, Peng Fyn Wu, Daniel Robinson, Hsing-Jien Kung, Chin Wen Chi, Chew Wun Wu, Wen Kang Yang, Jacqueline J K Whang-Peng, Wen Chang Lin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or vital factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain- reaction products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.

Original languageEnglish (US)
Pages (from-to)101-110
Number of pages10
JournalJournal of Biomedical Science
Volume5
Issue number2
StatePublished - 1998
Externally publishedYes

Fingerprint

Protein-Serine-Threonine Kinases
Protein-Tyrosine Kinases
Protein Kinases
Stomach Neoplasms
Cells
Cell Line
Tumors
Genes
Polymerase chain reaction
Polymerase Chain Reaction
Neoplasms
Genetic Vectors
Signal transduction
Gene Amplification
Neoplasm Staging
Cloning
Reaction products
Cell Division
Amplification
Sequence Analysis

Keywords

  • Gastric cancer
  • Protein serine/threonine kinase
  • Protein tyrosine kinase
  • RT-PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lin, J. S., Lu, C. W., Huang, C. J., Wu, P. F., Robinson, D., Kung, H-J., ... Lin, W. C. (1998). Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines. Journal of Biomedical Science, 5(2), 101-110.

Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines. / Lin, Jyh Shi; Lu, Chi Wei; Huang, Chang Jen; Wu, Peng Fyn; Robinson, Daniel; Kung, Hsing-Jien; Chi, Chin Wen; Wu, Chew Wun; Yang, Wen Kang; Whang-Peng, Jacqueline J K; Lin, Wen Chang.

In: Journal of Biomedical Science, Vol. 5, No. 2, 1998, p. 101-110.

Research output: Contribution to journalArticle

Lin, JS, Lu, CW, Huang, CJ, Wu, PF, Robinson, D, Kung, H-J, Chi, CW, Wu, CW, Yang, WK, Whang-Peng, JJK & Lin, WC 1998, 'Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines', Journal of Biomedical Science, vol. 5, no. 2, pp. 101-110.
Lin, Jyh Shi ; Lu, Chi Wei ; Huang, Chang Jen ; Wu, Peng Fyn ; Robinson, Daniel ; Kung, Hsing-Jien ; Chi, Chin Wen ; Wu, Chew Wun ; Yang, Wen Kang ; Whang-Peng, Jacqueline J K ; Lin, Wen Chang. / Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines. In: Journal of Biomedical Science. 1998 ; Vol. 5, No. 2. pp. 101-110.
@article{6fc53e68c0164462a9532029d7c91459,
title = "Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines",
abstract = "Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or vital factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain- reaction products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.",
keywords = "Gastric cancer, Protein serine/threonine kinase, Protein tyrosine kinase, RT-PCR",
author = "Lin, {Jyh Shi} and Lu, {Chi Wei} and Huang, {Chang Jen} and Wu, {Peng Fyn} and Daniel Robinson and Hsing-Jien Kung and Chi, {Chin Wen} and Wu, {Chew Wun} and Yang, {Wen Kang} and Whang-Peng, {Jacqueline J K} and Lin, {Wen Chang}",
year = "1998",
language = "English (US)",
volume = "5",
pages = "101--110",
journal = "Journal of Biomedical Science",
issn = "1021-7770",
publisher = "BioMed Central",
number = "2",

}

TY - JOUR

T1 - Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines

AU - Lin, Jyh Shi

AU - Lu, Chi Wei

AU - Huang, Chang Jen

AU - Wu, Peng Fyn

AU - Robinson, Daniel

AU - Kung, Hsing-Jien

AU - Chi, Chin Wen

AU - Wu, Chew Wun

AU - Yang, Wen Kang

AU - Whang-Peng, Jacqueline J K

AU - Lin, Wen Chang

PY - 1998

Y1 - 1998

N2 - Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or vital factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain- reaction products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.

AB - Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or vital factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain- reaction products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.

KW - Gastric cancer

KW - Protein serine/threonine kinase

KW - Protein tyrosine kinase

KW - RT-PCR

UR - http://www.scopus.com/inward/record.url?scp=7144263746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7144263746&partnerID=8YFLogxK

M3 - Article

C2 - 9662069

AN - SCOPUS:7144263746

VL - 5

SP - 101

EP - 110

JO - Journal of Biomedical Science

JF - Journal of Biomedical Science

SN - 1021-7770

IS - 2

ER -