Protein targets of 1,4-benzoquinone and 1,4-naphthoquinone in human bronchial epithelial cells

Michael W. Lamé, A. Daniel Jones, Dennis W Wilson, Hank J. Segall

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Many aspects of the toxicity of xenobiotic compounds have been attributed to the consequences of covalent modification of specific proteins, but the nature and specificity of protein targets for classes of electrophilic toxins remain largely uncharacterized. For inhaled toxicants, the point of exposure or absorption lies with epithelial cells lining the pulmonary tree. In this study, abundant proteins in human bronchial epithelial cells that are arylated in vitro by two quinonoid compounds, 1,4-benzoquinone (BQ) and 1,4-naphthoquinone (NQ) have been detected using 14C-labeled quinones and two-dimensional gel electrophoresis. These proteins were identified using matrix assisted laser desorption/ionization mass spectrometry for tryptic mass mapping followed by sequence database searching. Corroborative identification of protein targets was obtained from the apparent isoelectric points, molecular weights, and the use of antibody probes. There were subtle differences in the protein targets of BQ and NQ, but both associated with the following abundant proteins, nucleophosmin, galectin-1, probable protein disulfide isomerase, protein disulfide isomerase, 60 kDa heat shock protein, mitochondrial stress-70 protein, epithelial cell marker protein, and S100-type calcium binding protein A14. We further delineate the properties of these proteins that make them preferred targets and the evidence these adducts present for delivery of these quinones to subcellular compartments.

Original languageEnglish (US)
Pages (from-to)479-495
Number of pages17
JournalProteomics
Volume3
Issue number4
DOIs
StatePublished - Apr 1 2003

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Epithelial Cells
Proteins
Protein Disulfide-Isomerases
Quinones
Galectin 1
Chaperonin 60
Calcium-Binding Proteins
S100 Proteins
1,4-naphthoquinone
benzoquinone
Mitochondrial Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Isoelectric Point
Xenobiotics
Heat-Shock Proteins
Electrophoresis
Linings
Ionization
Mass spectrometry

Keywords

  • Benzoquinone
  • Human bronchial epithelial cells
  • Naphthoquinone
  • Protein adducts
  • Reactive cysteine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Protein targets of 1,4-benzoquinone and 1,4-naphthoquinone in human bronchial epithelial cells. / Lamé, Michael W.; Jones, A. Daniel; Wilson, Dennis W; Segall, Hank J.

In: Proteomics, Vol. 3, No. 4, 01.04.2003, p. 479-495.

Research output: Contribution to journalArticle

Lamé, Michael W. ; Jones, A. Daniel ; Wilson, Dennis W ; Segall, Hank J. / Protein targets of 1,4-benzoquinone and 1,4-naphthoquinone in human bronchial epithelial cells. In: Proteomics. 2003 ; Vol. 3, No. 4. pp. 479-495.
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