Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes

J. P. Landry, Y. S. Sun, X. W. Guo, X. D. Zhu

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

We applied oblique-incidence reflectivity difference microscopes (a form of polarization-modulated nulling ellipsometry) to detection of biomolecular microarrays without external labeling in a study of protein reactions with surface-immobilized targets. We show that the optical reflectivity difference signals can be quantitatively related to changes in surface mass density of molecular layers as a result of the reactions. Our experimental results demonstrate the feasibility of using oblique-incidence reflectivity difference microscopes for high-throughput proteomics research such as screening unlabeled protein probes against libraries of surface-immobilized small molecules.

Original languageEnglish (US)
Pages (from-to)3275-3288
Number of pages14
JournalApplied Optics
Volume47
Issue number18
DOIs
StatePublished - Jun 20 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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