Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe

Kazuhiro Shiozaki, Haleh Akhavan-Niaki, Clare H. McGowan, Paul Russell

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.

Original languageEnglish (US)
Pages (from-to)3742-3751
Number of pages10
JournalMolecular and Cellular Biology
Volume14
Issue number6
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Heat-Shock Response
Schizosaccharomyces
Phosphoprotein Phosphatases
Shock
Enzymes
Hot Temperature
Suppressor Genes
Temperature
Threonine
Multigene Family
Heat-Shock Proteins
Protein Phosphatase 2C
Phosphoric Monoester Hydrolases
Serine
Genes
Proteins
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe. / Shiozaki, Kazuhiro; Akhavan-Niaki, Haleh; McGowan, Clare H.; Russell, Paul.

In: Molecular and Cellular Biology, Vol. 14, No. 6, 06.1994, p. 3742-3751.

Research output: Contribution to journalArticle

Shiozaki, K, Akhavan-Niaki, H, McGowan, CH & Russell, P 1994, 'Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe', Molecular and Cellular Biology, vol. 14, no. 6, pp. 3742-3751.
Shiozaki, Kazuhiro ; Akhavan-Niaki, Haleh ; McGowan, Clare H. ; Russell, Paul. / Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 6. pp. 3742-3751.
@article{dc11b1fb3cdc443280e0a490d8ed0f4c,
title = "Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe",
abstract = "Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24{\%} identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.",
author = "Kazuhiro Shiozaki and Haleh Akhavan-Niaki and McGowan, {Clare H.} and Paul Russell",
year = "1994",
month = "6",
language = "English (US)",
volume = "14",
pages = "3742--3751",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe

AU - Shiozaki, Kazuhiro

AU - Akhavan-Niaki, Haleh

AU - McGowan, Clare H.

AU - Russell, Paul

PY - 1994/6

Y1 - 1994/6

N2 - Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.

AB - Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.

UR - http://www.scopus.com/inward/record.url?scp=0028298227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028298227&partnerID=8YFLogxK

M3 - Article

C2 - 8196617

AN - SCOPUS:0028298227

VL - 14

SP - 3742

EP - 3751

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 6

ER -