Abstract
Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3742-3751 |
| Number of pages | 10 |
| Journal | Molecular and Cellular Biology |
| Volume | 14 |
| Issue number | 6 |
| State | Published - Jun 1994 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
- Genetics
- Molecular Biology
Cite this
Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe. / Shiozaki, Kazuhiro; Akhavan-Niaki, Haleh; McGowan, Clare H.; Russell, Paul.
In: Molecular and Cellular Biology, Vol. 14, No. 6, 06.1994, p. 3742-3751.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein phosphatase 2C, encoded by ptc1+, is important in the heat shock response of Schizosaccharomyces pombe
AU - Shiozaki, Kazuhiro
AU - Akhavan-Niaki, Haleh
AU - McGowan, Clare H.
AU - Russell, Paul
PY - 1994/6
Y1 - 1994/6
N2 - Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.
AB - Protein phosphatase 2C (PP2C), an Mg2+-dependent enzyme that dephosphorylates serine and threonine residues, defines one of the three major families of structurally unrelated eukaryotic protein phosphatases. Members of the two other families of protein phosphatases are known to have important cellular roles, but very little is known about the biological functions of PP2C. In this report we describe a genetic investigation of a PP2C enzyme in the fission yeast Schizosaccharomyces pombe. We discovered ptc1+ (phosphatase two C) as a multicopy suppressor gene of swo1-26, a temperature-sensitive mutation of a gene encoding the heat shock protein hsp90. The ptc1+ gene product is a 40-kDa protein with ~24% identity to a rat PP2C protein. Purified Ptc1 has Mg2+-dependent casein phosphatase activity, confirming that it is a PP2C enzyme. A ptc1 deletion mutant is viable and has approximately normal levels of PP2C activity, observations consistent with the fact that ptc1+ is a member of a multigene family. Although a ptc1 deletion mutant is viable, it has a greatly reduced ability to survive brief exposure to elevated temperature. Moreover, ptc1+ mRNA levels increase 5- to 10-fold during heat shock. These data, demonstrating that Ptc1 activity is important for survival of heat shock, provide one of the first genetic clues as to the biological functions of PP2C.
UR - http://www.scopus.com/inward/record.url?scp=0028298227&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028298227&partnerID=8YFLogxK
M3 - Article
C2 - 8196617
AN - SCOPUS:0028298227
VL - 14
SP - 3742
EP - 3751
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 6
ER -