Protein Kinase C Is Not Involved in Ah Receptor Transformation and DNA Binding

M. W. Schafer, B. V. Madhukar, H. I. Swanson, K. Tullis, M. S. Denison

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Induction of cytochrome P4501A1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is mediated by the Ah receptor (AhR) complex, a ligand-dependent DNA-binding transactivator. Recently a role for protein kinase C (PKC) in the induction response has been reported in which PKC or a related kinase positively modulates AhR activity. We have examined the role of PKC by determining the effect of two nonspecific PKC inhibitors, H7 and staurosporine, and one specific PKC inhibitor, calphostin c, on AhR functionality. Although no kinase activity was detectable in cytosol, under the conditions used for our assays, AhR transformation and DNA binding still occurred. Addition of relatively high concentrations of the kinase inhibitors also had no significant effect on TCDD:AhR:DRE complex formation. Thus, our results indicate that protein kinase activity does not appear to be necessary for TCDD-dependent AhR transformation and DNA binding and they imply that protein kinases must play a role in another step(s) in the AhR-dependent mechanism of P4501A1 induction.

Original languageEnglish (US)
Pages (from-to)267-271
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume307
Issue number2
DOIs
StatePublished - Dec 1993

Fingerprint

Protein Kinase C
DNA
Phosphotransferases
Protein C Inhibitor
Protein Kinase Inhibitors
Protein Kinases
Aryl Hydrocarbon Receptors
Trans-Activators
Staurosporine
DNA-Binding Proteins
Cytochromes
Cytosol
Assays
DNA receptor
Ligands
1,4-dioxin
Polychlorinated Dibenzodioxins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Schafer, M. W., Madhukar, B. V., Swanson, H. I., Tullis, K., & Denison, M. S. (1993). Protein Kinase C Is Not Involved in Ah Receptor Transformation and DNA Binding. Archives of Biochemistry and Biophysics, 307(2), 267-271. https://doi.org/10.1006/abbi.1993.1589

Protein Kinase C Is Not Involved in Ah Receptor Transformation and DNA Binding. / Schafer, M. W.; Madhukar, B. V.; Swanson, H. I.; Tullis, K.; Denison, M. S.

In: Archives of Biochemistry and Biophysics, Vol. 307, No. 2, 12.1993, p. 267-271.

Research output: Contribution to journalArticle

Schafer, MW, Madhukar, BV, Swanson, HI, Tullis, K & Denison, MS 1993, 'Protein Kinase C Is Not Involved in Ah Receptor Transformation and DNA Binding', Archives of Biochemistry and Biophysics, vol. 307, no. 2, pp. 267-271. https://doi.org/10.1006/abbi.1993.1589
Schafer, M. W. ; Madhukar, B. V. ; Swanson, H. I. ; Tullis, K. ; Denison, M. S. / Protein Kinase C Is Not Involved in Ah Receptor Transformation and DNA Binding. In: Archives of Biochemistry and Biophysics. 1993 ; Vol. 307, No. 2. pp. 267-271.
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