Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts

Yu-Fung Lin, Michael D. Browning, Ellen M. Dudek, Robert L. Macdonald

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The β1 and γ2L subunits of the γ-aminobutyric acid type A receptor (GABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on GABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant α1β1γ2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular application of PKM increased GABAR currents, and the enhancement was antagonized by coapplication of the PKC inhibitory peptide. No acceleration or deceleration of GABAR desensitization was observed. The second experiment was a reimpalement study in which paired recordings were made successively from individual cells. Enhancement of GABAR currents by PKM was again obtained. PKM increased GABAR currents at high (>10 μM) but not at low (<10 μM) GABA concentrations, resulting in increases in both EC50 and maximal GABAR current. Thus, PKC phosphorylation enhanced recombinant α1β1γ2L GABAR current by increasing maximal current without increasing the affinity of GABA for the GABAAs.

Original languageEnglish (US)
Pages (from-to)1421-1431
Number of pages11
JournalNeuron
Volume13
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

GABA-A Receptors
gamma-Aminobutyric Acid
Protein Kinase C
Fibroblasts
Phosphorylation
Aminobutyrates
Deceleration
Patch-Clamp Techniques
Peptides
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts. / Lin, Yu-Fung; Browning, Michael D.; Dudek, Ellen M.; Macdonald, Robert L.

In: Neuron, Vol. 13, No. 6, 1994, p. 1421-1431.

Research output: Contribution to journalArticle

Lin, Yu-Fung ; Browning, Michael D. ; Dudek, Ellen M. ; Macdonald, Robert L. / Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts. In: Neuron. 1994 ; Vol. 13, No. 6. pp. 1421-1431.
@article{af78380c3a554ecb9773298ac9fa73e8,
title = "Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts",
abstract = "The β1 and γ2L subunits of the γ-aminobutyric acid type A receptor (GABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on GABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant α1β1γ2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular application of PKM increased GABAR currents, and the enhancement was antagonized by coapplication of the PKC inhibitory peptide. No acceleration or deceleration of GABAR desensitization was observed. The second experiment was a reimpalement study in which paired recordings were made successively from individual cells. Enhancement of GABAR currents by PKM was again obtained. PKM increased GABAR currents at high (>10 μM) but not at low (<10 μM) GABA concentrations, resulting in increases in both EC50 and maximal GABAR current. Thus, PKC phosphorylation enhanced recombinant α1β1γ2L GABAR current by increasing maximal current without increasing the affinity of GABA for the GABAAs.",
author = "Yu-Fung Lin and Browning, {Michael D.} and Dudek, {Ellen M.} and Macdonald, {Robert L.}",
year = "1994",
doi = "10.1016/0896-6273(94)90427-8",
language = "English (US)",
volume = "13",
pages = "1421--1431",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts

AU - Lin, Yu-Fung

AU - Browning, Michael D.

AU - Dudek, Ellen M.

AU - Macdonald, Robert L.

PY - 1994

Y1 - 1994

N2 - The β1 and γ2L subunits of the γ-aminobutyric acid type A receptor (GABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on GABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant α1β1γ2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular application of PKM increased GABAR currents, and the enhancement was antagonized by coapplication of the PKC inhibitory peptide. No acceleration or deceleration of GABAR desensitization was observed. The second experiment was a reimpalement study in which paired recordings were made successively from individual cells. Enhancement of GABAR currents by PKM was again obtained. PKM increased GABAR currents at high (>10 μM) but not at low (<10 μM) GABA concentrations, resulting in increases in both EC50 and maximal GABAR current. Thus, PKC phosphorylation enhanced recombinant α1β1γ2L GABAR current by increasing maximal current without increasing the affinity of GABA for the GABAAs.

AB - The β1 and γ2L subunits of the γ-aminobutyric acid type A receptor (GABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on GABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant α1β1γ2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular application of PKM increased GABAR currents, and the enhancement was antagonized by coapplication of the PKC inhibitory peptide. No acceleration or deceleration of GABAR desensitization was observed. The second experiment was a reimpalement study in which paired recordings were made successively from individual cells. Enhancement of GABAR currents by PKM was again obtained. PKM increased GABAR currents at high (>10 μM) but not at low (<10 μM) GABA concentrations, resulting in increases in both EC50 and maximal GABAR current. Thus, PKC phosphorylation enhanced recombinant α1β1γ2L GABAR current by increasing maximal current without increasing the affinity of GABA for the GABAAs.

UR - http://www.scopus.com/inward/record.url?scp=0028569777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028569777&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90427-8

DO - 10.1016/0896-6273(94)90427-8

M3 - Article

C2 - 7993633

AN - SCOPUS:0028569777

VL - 13

SP - 1421

EP - 1431

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 6

ER -