Protein kinase C enhances recombinant bovine α1β1γ2L GABAA receptor whole-cell currents expressed in L929 fibroblasts

Yu-Fung Lin, Michael D. Browning, Ellen M. Dudek, Robert L. Macdonald

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

The β1 and γ2L subunits of the γ-aminobutyric acid type A receptor (GABAR) contain phosphorylation sites for PKC. To determine the effect of PKC on GABAR function, whole-cell recordings were obtained from mouse fibroblasts expressing recombinant α1β1γ2L receptors, and catalytically active PKC (PKM) was applied via the recording pipette. The first experiment was a population study. Intracellular application of PKM increased GABAR currents, and the enhancement was antagonized by coapplication of the PKC inhibitory peptide. No acceleration or deceleration of GABAR desensitization was observed. The second experiment was a reimpalement study in which paired recordings were made successively from individual cells. Enhancement of GABAR currents by PKM was again obtained. PKM increased GABAR currents at high (>10 μM) but not at low (<10 μM) GABA concentrations, resulting in increases in both EC50 and maximal GABAR current. Thus, PKC phosphorylation enhanced recombinant α1β1γ2L GABAR current by increasing maximal current without increasing the affinity of GABA for the GABAAs.

Original languageEnglish (US)
Pages (from-to)1421-1431
Number of pages11
JournalNeuron
Volume13
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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