Protein-DNA interactions at recognition sites for the dioxin-Ah receptor complex

M. S. Denison, J. M. Fisher, J. P. Whitlock

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Gel retardation analyses reveal a cluster of six binding sites for the liganded Ah receptor within a 700-base pair DNA domain upstream of the mouse CYP 1A1 gene. The nucleotide sequences of the binding sites define a consensus recognition motif for the liganded receptor. The consensus motif is not symmetric. Alteration of the consensus motif produces a decrease in the receptor-DNA interaction. The liganded receptor binds as a monomer to its recognition motif and preferentially binds to double-stranded DNA. These observations reveal apparent differences between 2,3,7,8-tetrachlorodibenzo-p-dioxin and steroid hormones in their respective mechanisms of action.

Original languageEnglish (US)
Pages (from-to)16478-16482
Number of pages5
JournalJournal of Biological Chemistry
Volume264
Issue number28
StatePublished - 1989
Externally publishedYes

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Aryl Hydrocarbon Receptors
Dioxins
Steroid hormones
Binding Sites
Cytochrome P-450 CYP1A1
DNA
Base Pairing
Proteins
Nucleotides
Genes
Monomers
Gels
Steroids
Hormones
1,4-dioxin
DNA receptor
Polychlorinated Dibenzodioxins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Denison, M. S., Fisher, J. M., & Whitlock, J. P. (1989). Protein-DNA interactions at recognition sites for the dioxin-Ah receptor complex. Journal of Biological Chemistry, 264(28), 16478-16482.

Protein-DNA interactions at recognition sites for the dioxin-Ah receptor complex. / Denison, M. S.; Fisher, J. M.; Whitlock, J. P.

In: Journal of Biological Chemistry, Vol. 264, No. 28, 1989, p. 16478-16482.

Research output: Contribution to journalArticle

Denison, MS, Fisher, JM & Whitlock, JP 1989, 'Protein-DNA interactions at recognition sites for the dioxin-Ah receptor complex', Journal of Biological Chemistry, vol. 264, no. 28, pp. 16478-16482.
Denison, M. S. ; Fisher, J. M. ; Whitlock, J. P. / Protein-DNA interactions at recognition sites for the dioxin-Ah receptor complex. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 28. pp. 16478-16482.
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