Protein-DNA charge transport: Redox activation of a DNA repair protein by guanine radical

Eylon Yavin, Amie K. Boal, Eric D A Stemp, Elisabeth M. Boon, Alison L. Livingston, Valerie L. O'Shea, Sheila S. David, Jacqueline K. Barton

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

DNA charge transport (CT) chemistry provides a route to carry out oxidative DNA damage from a distance in a reaction that is sensitive to DNA mismatches and lesions. Here, DNA-mediated CT also leads to oxidation of a DNA-bound base excision repair enzyme, MutY. DNA-bound Ru(III), generated through a flash/ quench technique, is found to promote oxidation of the [4Fe-4S]2+ cluster of MutY to [4Fe-4S]3+ and its decomposition product [3Fe-4S]1+. Flash/quench experiments monitored by EPR spectroscopy reveal spectra with g = 2.08, 2.06, and 2.02, characteristic of the oxidized clusters. Transient absorption spectra of poly(dGC) and [Ru(phen) 2dppz]3+ (dppz = dipyridophenazine), generated in situ, show an absorption characteristic of the guanine radical that is depleted in the presence of MutY with formation instead of a long-lived species with an absorption at 405 nm; we attribute this absorption also to formation of the oxidized [4Fe-4S]3+ and [3Fe-4S]1+ clusters. In ruthenium-tethered DNA assemblies, oxidative damage to the 5′-G of a 5′-GG-3′ doublet is generated from a distance but this irreversible damage is inhibited by MutY and instead EPR experiments reveal cluster oxidation. With ruthenium-tethered assemblies containing duplex versus single-stranded regions, MutY oxidation is found to be mediated by the DNA duplex, with guanine radical as an intermediate oxidant; guanine radical formation facilitates MutY oxidation. A model is proposed for the redox activation of DNA repair proteins through DNA CT, with guanine radicals, the first product under oxidative stress, in oxidizing the DNA-bound repair proteins, providing the signal to stimulate DNA repair.

Original languageEnglish (US)
Pages (from-to)3546-3551
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number10
DOIs
StatePublished - Mar 8 2005
Externally publishedYes

Keywords

  • Electron transfer
  • Iron-sulfur cluster
  • Oxidative DNA damage

ASJC Scopus subject areas

  • Genetics
  • General

Fingerprint Dive into the research topics of 'Protein-DNA charge transport: Redox activation of a DNA repair protein by guanine radical'. Together they form a unique fingerprint.

  • Cite this

    Yavin, E., Boal, A. K., Stemp, E. D. A., Boon, E. M., Livingston, A. L., O'Shea, V. L., David, S. S., & Barton, J. K. (2005). Protein-DNA charge transport: Redox activation of a DNA repair protein by guanine radical. Proceedings of the National Academy of Sciences of the United States of America, 102(10), 3546-3551. https://doi.org/10.1073/pnas.0409410102