Protein conformation and diagnostic tests

The prion protein

Brian J. Bennionn, Valerie Daggett

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Many clinical diagnostic tests depend on the accurate detection and quantification of proteins and peptides and their functions. Alterations of protein structure, and the resulting consequences on dynamics, can affect the outcome of laboratory tests. These changes can be a result of mutations, other in vivo factors, or even the experimental conditions of the diagnostic test. Approach: The relationship between protein structure and dynamics and experimentally observable properties used in diagnostic assays are discussed in light of transmissible spongiform encephalopathies, or prion diseases. Content: This review describes current efforts and possible future directions of prion diagnostic development. Recent advances in therapeutic development are also addressed. Summary: The intent of the review is to highlight the role of protein dynamics and conformational change in protein-based diagnostics and treatments for prion disease.

Original languageEnglish (US)
Pages (from-to)2105-2114
Number of pages10
JournalClinical Chemistry
Volume48
Issue number12
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Protein Conformation
Routine Diagnostic Tests
Conformations
Prion Diseases
Prions
Proteins
Assays
Prion Proteins
Peptides
Mutation

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Bennionn, B. J., & Daggett, V. (2002). Protein conformation and diagnostic tests: The prion protein. Clinical Chemistry, 48(12), 2105-2114.

Protein conformation and diagnostic tests : The prion protein. / Bennionn, Brian J.; Daggett, Valerie.

In: Clinical Chemistry, Vol. 48, No. 12, 01.12.2002, p. 2105-2114.

Research output: Contribution to journalArticle

Bennionn, BJ & Daggett, V 2002, 'Protein conformation and diagnostic tests: The prion protein', Clinical Chemistry, vol. 48, no. 12, pp. 2105-2114.
Bennionn BJ, Daggett V. Protein conformation and diagnostic tests: The prion protein. Clinical Chemistry. 2002 Dec 1;48(12):2105-2114.
Bennionn, Brian J. ; Daggett, Valerie. / Protein conformation and diagnostic tests : The prion protein. In: Clinical Chemistry. 2002 ; Vol. 48, No. 12. pp. 2105-2114.
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