Protein and nutrient composition of dog milk throughout lactation

A. J. Lepine, Y. Adkins, B. Lönnerdal

Research output: Contribution to journalArticle

Abstract

There is limited knowledge about the protein and nutrient composition of dog milk and how it changes during lactation. In this study, milk samples were collected on days 1, 3, 7, 14, 28, 35, and 42 from 10 beagle dogs. Samples were analyzed for nitrogen, non-protein nitrogen (NPN), protein and casein/whey ratio by a micro-Kjeldahl method and precipitation at pH 4.6, lipids and fatty acids by extraction and gas chromatography, lactose by a lactase assay, citrate by a commercial assay and minerals and trace elements by atomic absorption spectrometry. Protein concentration was very high in day 1 samples (144±60 g/L) then decreased through day 21 (68±13 g/L); a modest increase was then observed. There was no change in the concentration of NPN (approx. 6-10% of total N). The casein/whey ratio was about 70/30 and stayed constant throughout lactation. Lactose concentration increased during early lactation; dl samples contained 17±4 g/L, while d7 to d42 samples contained 34-40 g/L. Lipid concentration was high in early lactation (approx. 13%) and then decreased slighly (approx. 11%). Citrate concentration increased from d1 to d7 (4.8±1.2 to 6.6±1.0 mM), then gradually decreased by d42 (3.9±1.2 mM). These data will be valuable when establishing the nutrient requirements of puppies during the suckling period.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

Fingerprint

protein composition
Lactation
Nutrients
Milk
nutrient content
Nitrogen
lactation
Dogs
Lactose
Caseins
Citric Acid
milk
Food
dogs
Assays
Chemical analysis
nonprotein nitrogen
Lactase
Lipids
early lactation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Lepine, A. J., Adkins, Y., & Lönnerdal, B. (1998). Protein and nutrient composition of dog milk throughout lactation. FASEB Journal, 12(5).

Protein and nutrient composition of dog milk throughout lactation. / Lepine, A. J.; Adkins, Y.; Lönnerdal, B.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Lepine, AJ, Adkins, Y & Lönnerdal, B 1998, 'Protein and nutrient composition of dog milk throughout lactation', FASEB Journal, vol. 12, no. 5.
Lepine AJ, Adkins Y, Lönnerdal B. Protein and nutrient composition of dog milk throughout lactation. FASEB Journal. 1998 Mar 20;12(5).
Lepine, A. J. ; Adkins, Y. ; Lönnerdal, B. / Protein and nutrient composition of dog milk throughout lactation. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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