Protein and antigen profiles of prevalent serovars of Leptospira interrogans

J. A. Brown, Rance B Lefebvre, Jeng Pan Ming Jeng Pan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Whole-cell and detergent-soluble proteins, enriched for outer membrane antigens, of the Leptospira interrogans serovars present in commercially available pentavalent vaccines (hardjo, pomona, icterohaemorrhagiae, grippotyphosa, and canicola) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (immunoblotting). Protein and antigenic profiles of these serovars, representing several serogroups, were compared with similar profiles of the most common North American bovine pathogen, serovar hardjo type hardjo bovis. The reference strain of serovar balcanica and a hardjoprajitno bovine field isolate (serovar hardjo) were also assayed. Coomassie blue-stained gels revealed extensive protein similarities, and Western blots demonstrated antigenic relatedness throughout the low- and high-molecular-weight regions. Possible quantitative differences in protein expression among the strains were noted, as were similarities in the protein profiles of type hardjo bovis and the balcanica reference strain. A cocktail composed of these homologous antigens may serve as an efficacious subunit vaccine for leptospirosis.

Original languageEnglish (US)
Pages (from-to)1772-1777
Number of pages6
JournalInfection and Immunity
Volume59
Issue number5
StatePublished - 1991

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Leptospira interrogans
Antigens
Proteins
Western Blotting
Leptospirosis
Subunit Vaccines
Sodium Dodecyl Sulfate
Detergents
Serogroup
Polyacrylamide Gel Electrophoresis
Vaccines
Molecular Weight
Gels
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Protein and antigen profiles of prevalent serovars of Leptospira interrogans. / Brown, J. A.; Lefebvre, Rance B; Ming Jeng Pan, Jeng Pan.

In: Infection and Immunity, Vol. 59, No. 5, 1991, p. 1772-1777.

Research output: Contribution to journalArticle

Brown, J. A. ; Lefebvre, Rance B ; Ming Jeng Pan, Jeng Pan. / Protein and antigen profiles of prevalent serovars of Leptospira interrogans. In: Infection and Immunity. 1991 ; Vol. 59, No. 5. pp. 1772-1777.
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