Protection of mice from lyme borreliosis by oral vaccination with Escherichia coli expressing OspA

Erol Fikrig, Stephen W Barthold, Fred S. Kantor, Richard A. Flavell

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Mice immunized with recombinant outer surface protein A (OspA) in Freund's adjuvant or with intraperitoneal injections of live Escherichia coli expressing OspA have been shown to be protected from infection with Borrelia burgdorferi. To investigate the efficacy of oral vaccination, C3H/He mice were inoculated with 108 live E. coli expressing recombinant OspA by gavage and boosted in a similar manner on days 10, 20, 30, and 40. The animals developed serum IgG antibodies to OspA by immunoblot and were protected from infection when challenged with 104 B. burgdorferi intradermally 14 days after the last boost. Control mice did not develop antibodies to OspA and were not protected against challenge infection. These results suggest that an oral preparation of recombinant OspA could potentially be used for vaccination.

Original languageEnglish (US)
Pages (from-to)1224-1227
Number of pages4
JournalJournal of Infectious Diseases
Volume164
Issue number6
StatePublished - Dec 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

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