Protection of human muscle acetylcholinesterase from soman by pyridostigmine bromide

Ricardo A Maselli, John D. Henderson, Jarae Ng, David Follette, Gregory Graves, Barry W. Wilson

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Introduction: Pretreatment with pyridostigmine bromide (PB) of human intercostal muscle fibers exposed to the irreversible acetylcholinesterase (AChE) inhibitor soman was investigated. Methods: Muscles were pretreated with 3 × 10-6 M PB or saline for 20 minutes, then exposed to 10 -7 M soman for 10 minutes. Results: AChE of muscles treated with soman alone was inhibited >95%. In contrast, PB pretreatment of soman-exposed bundles protected 20% of AChE activity. AChE of bundles exposed to PB alone recovered after 4 hours, but bundles exposed to both PB and soman did not. Soman-induced reduction of resting membrane potentials and increment of amplitudes and decay times of miniature endplate potentials (MEPPs) were partially corrected by PB pretreatment. Conclusions: In vitro pretreatment of human muscles with PB protected up to 20% of muscle AChE and ameliorated some deleterious effects on endplate physiology induced by soman.

Original languageEnglish (US)
Pages (from-to)591-595
Number of pages5
JournalMuscle and Nerve
Volume43
Issue number4
DOIs
StatePublished - Apr 2011

Keywords

  • AChE
  • Human muscle
  • MEPPs
  • Pyridostigmine bromide
  • Soman

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)
  • Physiology

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  • Cite this

    Maselli, R. A., Henderson, J. D., Ng, J., Follette, D., Graves, G., & Wilson, B. W. (2011). Protection of human muscle acetylcholinesterase from soman by pyridostigmine bromide. Muscle and Nerve, 43(4), 591-595. https://doi.org/10.1002/mus.21922