Protection from hypertension in mice by the Mediterranean diet is mediated by nitro fatty acid inhibition of soluble epoxide hydrolase

Rebecca L. Charles, Olena Rudyk, Oleksandra Prysyazhna, Alisa Kamynina, Jun Yang, Christophe Morisseau, Bruce D. Hammock, Bruce A. Freeman, Philip Eaton

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Soluble epoxide hydrolase (sEH) is inhibited by electrophilic lipids by their adduction to Cys521 proximal to its catalytic center. This inhibition prevents hydrolysis of the enzymes' epoxyeicosatrienoic acid (EET) substrates, so they accumulate inducing vasodilation to lower blood pressure (BP). We generated a Cys521Ser sEH redoxdead knockin (KI) mouse model that was resistant to this mode of inhibition. The electrophilic lipid 10-nitro-oleic acid (NO 2-OA) inhibited hydrolase activity and also lowered BP in an angiotensin II-induced hypertension model in wild-type (WT) but not KI mice. Furthermore, EET/dihydroxy-epoxyeicosatrienoic acid isomer ratios were elevated in plasma from WT but not KI mice following NO2-OA treatment, consistent with the redox-dead mutant being resistant to inhibition by lipid electrophiles. sEH was inhibited in WT mice fed linoleic acid and nitrite, key constituents of the Mediterranean diet that elevates electrophilic nitro fatty acid levels, whereas KIs were unaffected. These observations reveal that lipid electrophiles such as NO2-OA mediate antihypertensive signaling actions by inhibiting sEH and suggest a mechanism accounting for protection from hypertension afforded by the Mediterranean diet.

Original languageEnglish (US)
Pages (from-to)8167-8172
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number22
DOIs
StatePublished - Jun 3 2014

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Mediterranean Diet
Epoxide Hydrolases
Fatty Acids
Hypertension
Lipids
Acids
Blood Pressure
Hydrolases
Linoleic Acid
Enzymes
Nitrites
Vasodilation
Angiotensin II
Antihypertensive Agents
Oxidation-Reduction
Hydrolysis

Keywords

  • Cardiovascular
  • Thiol

ASJC Scopus subject areas

  • General

Cite this

Protection from hypertension in mice by the Mediterranean diet is mediated by nitro fatty acid inhibition of soluble epoxide hydrolase. / Charles, Rebecca L.; Rudyk, Olena; Prysyazhna, Oleksandra; Kamynina, Alisa; Yang, Jun; Morisseau, Christophe; Hammock, Bruce D.; Freeman, Bruce A.; Eaton, Philip.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 22, 03.06.2014, p. 8167-8172.

Research output: Contribution to journalArticle

Charles, Rebecca L. ; Rudyk, Olena ; Prysyazhna, Oleksandra ; Kamynina, Alisa ; Yang, Jun ; Morisseau, Christophe ; Hammock, Bruce D. ; Freeman, Bruce A. ; Eaton, Philip. / Protection from hypertension in mice by the Mediterranean diet is mediated by nitro fatty acid inhibition of soluble epoxide hydrolase. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 22. pp. 8167-8172.
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