Protamine sulfate precipitation

A new assay for the Ah receptor

Michael S. Denison, Jay Fine, Christopher F. Wilkinson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.

Original languageEnglish (US)
Pages (from-to)28-36
Number of pages9
JournalAnalytical Biochemistry
Volume142
Issue number1
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Protamines
Precipitates
Assays
Aryl Hydrocarbon Receptors
Liver
Cytosol
Rats
Proteins
1,4-dioxin
Polychlorinated Dibenzodioxins

Keywords

  • 2,3,7,8-TCDD
  • Ah
  • protamine
  • receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Protamine sulfate precipitation : A new assay for the Ah receptor. / Denison, Michael S.; Fine, Jay; Wilkinson, Christopher F.

In: Analytical Biochemistry, Vol. 142, No. 1, 1984, p. 28-36.

Research output: Contribution to journalArticle

Denison, Michael S. ; Fine, Jay ; Wilkinson, Christopher F. / Protamine sulfate precipitation : A new assay for the Ah receptor. In: Analytical Biochemistry. 1984 ; Vol. 142, No. 1. pp. 28-36.
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