Protamine sulfate precipitation: A new assay for the Ah receptor

Michael S. Denison; Jay Fine; Christopher F. Wilkinson

doi:10.1016/0003-2697(84)90512-8

Protamine sulfate precipitation: A new assay for the Ah receptor

Michael S. Denison, Jay Fine, Christopher F. Wilkinson

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[³H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [³H]TCDD nonspecifically.

Original language	English (US)
Pages (from-to)	28-36
Number of pages	9
Journal	Analytical Biochemistry
Volume	142
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(84)90512-8
State	Published - 1984
Externally published	Yes

Keywords

2,3,7,8-TCDD
Ah
protamine
receptor

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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abstract = "The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.",

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AU - Denison, Michael S.

AU - Fine, Jay

AU - Wilkinson, Christopher F.

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AB - The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.

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KW - Ah

KW - protamine

KW - receptor

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