Abstract
The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 28-36 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 142 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
| Externally published | Yes |
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Keywords
- 2,3,7,8-TCDD
- Ah
- protamine
- receptor
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Protamine sulfate precipitation : A new assay for the Ah receptor. / Denison, Michael S.; Fine, Jay; Wilkinson, Christopher F.
In: Analytical Biochemistry, Vol. 142, No. 1, 1984, p. 28-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protamine sulfate precipitation
T2 - A new assay for the Ah receptor
AU - Denison, Michael S.
AU - Fine, Jay
AU - Wilkinson, Christopher F.
PY - 1984
Y1 - 1984
N2 - The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.
AB - The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.
KW - 2,3,7,8-TCDD
KW - Ah
KW - protamine
KW - receptor
UR - http://www.scopus.com/inward/record.url?scp=0021748497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021748497&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(84)90512-8
DO - 10.1016/0003-2697(84)90512-8
M3 - Article
VL - 142
SP - 28
EP - 36
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -