Prostaglandins and thromboxane: tert.-Butyldimethylsilyl ethers as derivatives for their analyses by gas chromatography-mass spectrometry-selected ion monitoring

J. Mai, B. German, J. E. Kinsella

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

tert.-Butyldimethylsilyl ethers of eighteen prostaglandins (PGs) and thromboxane have been analyzed by combined packed-column or fused-silica capillary gas chromatography-mass spectrometry (GC-MS). Except for thromboxane B2 and PGs of the 3 series the [M- 57] ion, the loss from the molecular ion of the tert.-butyl radical, is generally the major peak in the spectra of PGs. This high abundance of large fragments provides suitable criteria for the employment of these derivatives in GC-MS selected ion monitoring for rapid quantity estimation of PGs from biological samples. PGs from rat stomach fundus were rapidly quantitated in a single chromatogram using this procedure.

Original languageEnglish (US)
Pages (from-to)91-107
Number of pages17
JournalJournal of Chromatography A
Volume254
Issue numberC
DOIs
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Fingerprint Dive into the research topics of 'Prostaglandins and thromboxane: tert.-Butyldimethylsilyl ethers as derivatives for their analyses by gas chromatography-mass spectrometry-selected ion monitoring'. Together they form a unique fingerprint.

  • Cite this