Abstract
Prostaglandin E2 (PGE2) was shown to mediate load-induced bone remodeling in vivo. In vitro, it was demonstrated that PGE2 production in osteoblastic cell lines increases in response to oscillatory fluid flow (OFF). PGE2 production was investigated whether it is dependent upon intracellular calcium concentration. An image and analysis package was used to quantify the signals. However, both lines responded to OFF with a significant relative increase in PGE2 production compared to the no flow controls with responses greater at 2Hz. Furthermore, the PGE2 response of ROS cells was greater than that of RC × 16 cells.
| Original language | English (US) |
|---|---|
| Journal | Annals of Biomedical Engineering |
| Volume | 28 |
| Issue number | SUPPL. 1 |
| State | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Prostaglandin E2 (PGE2) response to fluid flow is independent of intracellular calcium concentration in osteoblastic ROS 17/2.8 and RC×16 cells. / Saunders, Marnie; You, Jun; Yellowley-genetos, Clare E; Donahue, Henry; Jacobs, Christopher.
In: Annals of Biomedical Engineering, Vol. 28, No. SUPPL. 1, 2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prostaglandin E2 (PGE2) response to fluid flow is independent of intracellular calcium concentration in osteoblastic ROS 17/2.8 and RC×16 cells
AU - Saunders, Marnie
AU - You, Jun
AU - Yellowley-genetos, Clare E
AU - Donahue, Henry
AU - Jacobs, Christopher
PY - 2000
Y1 - 2000
N2 - Prostaglandin E2 (PGE2) was shown to mediate load-induced bone remodeling in vivo. In vitro, it was demonstrated that PGE2 production in osteoblastic cell lines increases in response to oscillatory fluid flow (OFF). PGE2 production was investigated whether it is dependent upon intracellular calcium concentration. An image and analysis package was used to quantify the signals. However, both lines responded to OFF with a significant relative increase in PGE2 production compared to the no flow controls with responses greater at 2Hz. Furthermore, the PGE2 response of ROS cells was greater than that of RC × 16 cells.
AB - Prostaglandin E2 (PGE2) was shown to mediate load-induced bone remodeling in vivo. In vitro, it was demonstrated that PGE2 production in osteoblastic cell lines increases in response to oscillatory fluid flow (OFF). PGE2 production was investigated whether it is dependent upon intracellular calcium concentration. An image and analysis package was used to quantify the signals. However, both lines responded to OFF with a significant relative increase in PGE2 production compared to the no flow controls with responses greater at 2Hz. Furthermore, the PGE2 response of ROS cells was greater than that of RC × 16 cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034516621&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0034516621
VL - 28
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
SN - 0090-6964
IS - SUPPL. 1
ER -