Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae

G. Foster, Robert Higgins, D. Leclair, B. M. Korczak, I. Mikaelian, I. A P Patterson, P. Kuhnert

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Phenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family Pasteurellaceae. 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family Pasteurellaceae with,<96% sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including rpoB, infB and recN genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from recN gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as Bisgaardia hudsonensis gen. nov., sp. nov. (the type species) and Bisgaardia genomospecies 1. The G+C content of the DNA was 39.5 mol%. The type strain of Bisgaardia hudsonensis gen. nov., sp. nov. is M327/99/2 T (=CCUG 43067 T=NCTC 13475 T598-D-690B T) and the reference strain of Bisgaardia genomospecies 1 is M1765/96/5 (5CCUG 595515NCTC 13474).

Original languageEnglish (US)
Pages (from-to)3016-3022
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume61
Issue number12
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

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Pasteurellaceae
seals
Bacteria
Multilocus Sequence Typing
Negative Staining
gene
new species
phylogeny
bacteria
Base Composition
rRNA Genes
Genes
phylogenetics
new genus
bacterium
genes
ribosomal RNA
Genome
relatedness
nucleotide sequences

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Proposal of Bisgaardia hudsonensis gen. nov., sp. nov. and an additional genomospecies, isolated from seals, as new members of the family Pasteurellaceae. / Foster, G.; Higgins, Robert; Leclair, D.; Korczak, B. M.; Mikaelian, I.; Patterson, I. A P; Kuhnert, P.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 61, No. 12, 01.12.2011, p. 3016-3022.

Research output: Contribution to journalArticle

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abstract = "Phenotypic and phylogenetic studies were performed on eight Gram-negative-staining, rod-shaped bacteria isolated from seals. Biochemical and physiological studies showed identical profiles for all of the isolates and indicated that they were related to the family Pasteurellaceae. 16S rRNA gene sequencing demonstrated that the organism represented a distinct cluster with two sublines within the family Pasteurellaceae with,<96{\%} sequence similarity to any recognized species. Multilocus sequence analysis (MLSA) including rpoB, infB and recN genes further confirmed these findings with the eight isolates forming a genus-like cluster with two branches. Genome relatedness as deduced from recN gene sequences suggested that the isolates represented a new genus with two species. On the basis of the results of the phylogenetic analysis and phenotypic criteria, it is proposed that these bacteria from seals are classified as Bisgaardia hudsonensis gen. nov., sp. nov. (the type species) and Bisgaardia genomospecies 1. The G+C content of the DNA was 39.5 mol{\%}. The type strain of Bisgaardia hudsonensis gen. nov., sp. nov. is M327/99/2 T (=CCUG 43067 T=NCTC 13475 T598-D-690B T) and the reference strain of Bisgaardia genomospecies 1 is M1765/96/5 (5CCUG 595515NCTC 13474).",
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