Proportion of CD4 and CD8 tumor infiltrating lymphocytes predicts survival in persistent/recurrent laryngeal squamous cell carcinoma

Rebecca Hoesli, Andrew C. Birkeland, Andrew J. Rosko, Mohamad Issa, Kelsey L. Chow, Nicole L. Michmerhuizen, Jacqueline E. Mann, Steven B. Chinn, Andrew G. Shuman, Mark E. Prince, Gregory T. Wolf, Carol R. Bradford, Jonathan B. McHugh, J. Chad Brenner, Matthew E. Spector

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Tumor infiltrating lymphocytes (TILs) have been shown to be an important prognostic factor in patients with previously untreated head and neck cancer. After organ preservation therapy for laryngeal cancer and subsequent persistence/recurrence, the prognostic value of TILs is unknown. Our goal was to determine if TILs have value as a prognostic biomarker in patients with surgically salvageable persistent/recurrent laryngeal squamous cell carcinoma. Levels of TILs were quantified on tissue microarrays from 183 patients undergoing salvage total laryngectomy for persistent/recurrent laryngeal cancer after radiation or chemoradiation between 1997 and 2014. Demographic and clinical data were abstracted. Immunohistology evaluation included CD4, CD8, PDL-1, p16, CD31, Vimentin, EGFR, and p53. Elevated levels of either CD8 or CD4 positive TILs were associated with improved disease specific survival (CD8: HR 0.46, 95% CI 0.24–0.88, CD4: HR 0.43; 95% CI 0.21–0.89) and disease free survival (CD8: HR 0.53, 95% CI 0.29–0.94, CD4: HR 0.52; 95% CI 0.27–0.99). Levels of CD8 (HR 0.74; 95% CI 0.47–1.17) or CD4 (HR 0.66; 95% CI 0.40–1.08) TILs were not significantly associated with overall survival. In bivariate analysis, patients with elevated CD4 and/or CD8 TILs had significantly improved disease specific survival (HR 0.42; 95% CI 0.21–0.83) and disease free survival (HR 0.45; 95% CI 0.24–0.84) compared to patients with low levels of CD4 and CD8. PDL-1, p16, CD31, Vimentin, EGFR, and p53 were not significant prognostic factors. On multivariate analysis, elevated CD8 TILs were associated with improved disease specific survival (HR 0.35; 95% CI 0.14–0.88, p =.02) and disease free survival (HR 0.41; 95% CI 0.17–0.96, p =.04). CD8, and possibly CD4, positive TILs are associated with favorable disease free and disease specific survival for recurrent/persistent laryngeal cancer.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalOral Oncology
Volume77
DOIs
StatePublished - Feb 2018
Externally publishedYes

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Tumor-Infiltrating Lymphocytes
Squamous Cell Carcinoma
Survival
Disease-Free Survival
Laryngeal Neoplasms
CD4-Positive T-Lymphocytes
Vimentin
Organ Preservation
Laryngectomy
Head and Neck Neoplasms
Multivariate Analysis
Biomarkers
Demography
Radiation
Recurrence

Keywords

  • CD4
  • CD8
  • Head and neck cancer
  • Laryngectomy
  • Prognosis
  • Recurrence
  • Salvage
  • Survival
  • Tumor infiltrating lymphocytes

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

Cite this

Proportion of CD4 and CD8 tumor infiltrating lymphocytes predicts survival in persistent/recurrent laryngeal squamous cell carcinoma. / Hoesli, Rebecca; Birkeland, Andrew C.; Rosko, Andrew J.; Issa, Mohamad; Chow, Kelsey L.; Michmerhuizen, Nicole L.; Mann, Jacqueline E.; Chinn, Steven B.; Shuman, Andrew G.; Prince, Mark E.; Wolf, Gregory T.; Bradford, Carol R.; McHugh, Jonathan B.; Brenner, J. Chad; Spector, Matthew E.

In: Oral Oncology, Vol. 77, 02.2018, p. 83-89.

Research output: Contribution to journalArticle

Hoesli, R, Birkeland, AC, Rosko, AJ, Issa, M, Chow, KL, Michmerhuizen, NL, Mann, JE, Chinn, SB, Shuman, AG, Prince, ME, Wolf, GT, Bradford, CR, McHugh, JB, Brenner, JC & Spector, ME 2018, 'Proportion of CD4 and CD8 tumor infiltrating lymphocytes predicts survival in persistent/recurrent laryngeal squamous cell carcinoma', Oral Oncology, vol. 77, pp. 83-89. https://doi.org/10.1016/j.oraloncology.2017.12.003
Hoesli, Rebecca ; Birkeland, Andrew C. ; Rosko, Andrew J. ; Issa, Mohamad ; Chow, Kelsey L. ; Michmerhuizen, Nicole L. ; Mann, Jacqueline E. ; Chinn, Steven B. ; Shuman, Andrew G. ; Prince, Mark E. ; Wolf, Gregory T. ; Bradford, Carol R. ; McHugh, Jonathan B. ; Brenner, J. Chad ; Spector, Matthew E. / Proportion of CD4 and CD8 tumor infiltrating lymphocytes predicts survival in persistent/recurrent laryngeal squamous cell carcinoma. In: Oral Oncology. 2018 ; Vol. 77. pp. 83-89.
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T1 - Proportion of CD4 and CD8 tumor infiltrating lymphocytes predicts survival in persistent/recurrent laryngeal squamous cell carcinoma

AU - Hoesli, Rebecca

AU - Birkeland, Andrew C.

AU - Rosko, Andrew J.

AU - Issa, Mohamad

AU - Chow, Kelsey L.

AU - Michmerhuizen, Nicole L.

AU - Mann, Jacqueline E.

AU - Chinn, Steven B.

AU - Shuman, Andrew G.

AU - Prince, Mark E.

AU - Wolf, Gregory T.

AU - Bradford, Carol R.

AU - McHugh, Jonathan B.

AU - Brenner, J. Chad

AU - Spector, Matthew E.

PY - 2018/2

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N2 - Tumor infiltrating lymphocytes (TILs) have been shown to be an important prognostic factor in patients with previously untreated head and neck cancer. After organ preservation therapy for laryngeal cancer and subsequent persistence/recurrence, the prognostic value of TILs is unknown. Our goal was to determine if TILs have value as a prognostic biomarker in patients with surgically salvageable persistent/recurrent laryngeal squamous cell carcinoma. Levels of TILs were quantified on tissue microarrays from 183 patients undergoing salvage total laryngectomy for persistent/recurrent laryngeal cancer after radiation or chemoradiation between 1997 and 2014. Demographic and clinical data were abstracted. Immunohistology evaluation included CD4, CD8, PDL-1, p16, CD31, Vimentin, EGFR, and p53. Elevated levels of either CD8 or CD4 positive TILs were associated with improved disease specific survival (CD8: HR 0.46, 95% CI 0.24–0.88, CD4: HR 0.43; 95% CI 0.21–0.89) and disease free survival (CD8: HR 0.53, 95% CI 0.29–0.94, CD4: HR 0.52; 95% CI 0.27–0.99). Levels of CD8 (HR 0.74; 95% CI 0.47–1.17) or CD4 (HR 0.66; 95% CI 0.40–1.08) TILs were not significantly associated with overall survival. In bivariate analysis, patients with elevated CD4 and/or CD8 TILs had significantly improved disease specific survival (HR 0.42; 95% CI 0.21–0.83) and disease free survival (HR 0.45; 95% CI 0.24–0.84) compared to patients with low levels of CD4 and CD8. PDL-1, p16, CD31, Vimentin, EGFR, and p53 were not significant prognostic factors. On multivariate analysis, elevated CD8 TILs were associated with improved disease specific survival (HR 0.35; 95% CI 0.14–0.88, p =.02) and disease free survival (HR 0.41; 95% CI 0.17–0.96, p =.04). CD8, and possibly CD4, positive TILs are associated with favorable disease free and disease specific survival for recurrent/persistent laryngeal cancer.

AB - Tumor infiltrating lymphocytes (TILs) have been shown to be an important prognostic factor in patients with previously untreated head and neck cancer. After organ preservation therapy for laryngeal cancer and subsequent persistence/recurrence, the prognostic value of TILs is unknown. Our goal was to determine if TILs have value as a prognostic biomarker in patients with surgically salvageable persistent/recurrent laryngeal squamous cell carcinoma. Levels of TILs were quantified on tissue microarrays from 183 patients undergoing salvage total laryngectomy for persistent/recurrent laryngeal cancer after radiation or chemoradiation between 1997 and 2014. Demographic and clinical data were abstracted. Immunohistology evaluation included CD4, CD8, PDL-1, p16, CD31, Vimentin, EGFR, and p53. Elevated levels of either CD8 or CD4 positive TILs were associated with improved disease specific survival (CD8: HR 0.46, 95% CI 0.24–0.88, CD4: HR 0.43; 95% CI 0.21–0.89) and disease free survival (CD8: HR 0.53, 95% CI 0.29–0.94, CD4: HR 0.52; 95% CI 0.27–0.99). Levels of CD8 (HR 0.74; 95% CI 0.47–1.17) or CD4 (HR 0.66; 95% CI 0.40–1.08) TILs were not significantly associated with overall survival. In bivariate analysis, patients with elevated CD4 and/or CD8 TILs had significantly improved disease specific survival (HR 0.42; 95% CI 0.21–0.83) and disease free survival (HR 0.45; 95% CI 0.24–0.84) compared to patients with low levels of CD4 and CD8. PDL-1, p16, CD31, Vimentin, EGFR, and p53 were not significant prognostic factors. On multivariate analysis, elevated CD8 TILs were associated with improved disease specific survival (HR 0.35; 95% CI 0.14–0.88, p =.02) and disease free survival (HR 0.41; 95% CI 0.17–0.96, p =.04). CD8, and possibly CD4, positive TILs are associated with favorable disease free and disease specific survival for recurrent/persistent laryngeal cancer.

KW - CD4

KW - CD8

KW - Head and neck cancer

KW - Laryngectomy

KW - Prognosis

KW - Recurrence

KW - Salvage

KW - Survival

KW - Tumor infiltrating lymphocytes

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