Propofol's effects on nociceptive behavior and spinal C-fos expression afer intraplantar formalin injection in with a mutation in the gamma-aminobutyric acid-typeA β3 subunit

Austin W. Merrill, Linda S Barter, Uwe Rudolph, Edmond I. Eger, Joseph F. Antognini, Mirela Iodi Carstens, Earl Carstens

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We investigated whether propofol affected nociceptive behavior and fos-like immunoreactivity (FLI) in the lumbo-sacral spinal cord after intraplantar formalin injection in wild-type (WT) mice and in mutant mice harboring a point mutation of the gamma-aminobutyric acid type A receptor, which renders them resistant to propofol. Bolus injection of propofol (30 mg/kg IV) in WT mice reduced phase 1 formalin-evoked behavior over the initial 2-3 min but did not alter phase 2 behavior or spinal FLI (64 ± 19 cells/section) compared with WT mice receiving intralipid vehicle plus intraplantar formalin (57 ± 19 cells/section). Most FLI was restricted to superficial dorsal horn laminae ipsilateral to the formalin injection. WT mice receiving a 60-min propofol infusion were anesthetized throughout and did not display nociceptive behavior but had FLI (58 ± 11 cells/section) that did not differ significantly from the other WT groups. Mutant mice receiving bolus injection of propofol (30 mg/kg) and intraplantar formalin were not anesthetized and exhibited nociceptive behavior. The total FLI in the spinal cord was 47 ± 29 cells/section. These data indicate that although propofol produces anesthesia, it does not prevent the FLI that is associated with nociception, a finding consistent with propofol lacking analgesic properties.

Original languageEnglish (US)
Pages (from-to)478-483
Number of pages6
JournalAnesthesia and Analgesia
Volume103
Issue number2
DOIs
StatePublished - Aug 2006

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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