Properties of iridoviruses from ornamental fish

Ronald Hedrick, TS S. McDowell

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

An iridovirus from 2 species of freshwater tropical imported fish was examined for its antigenic and biochemical properties and virulence for 3 species of food fish. The virus was found in both guppy (Poecilia reticulata) and doctor fish (Labroides dimidatus). The virus replicated in numerous fish cell lines including BF-2, CCO and EPC. Virus titers reached 108 TCID50/ml in each cell line. The virions had similar size and morphology to those previously reported to cause systemic infections among catfish in Europe and from redfin perch and rainbow trout in Australia. A comparison of the ornamental fish iridovirus with these systemic agents indicated that all share common antigens detected by indirect fluorescent antibody tests and Western blots. Additionally, polymerase chain reactions using primer sets developed for the red fin perch virus, amplified a segment of genomic DNA from the ornamental fish isolate. An examination of the virulence of the ornamental fish isolate for 2 important food fish species, rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus), indicated the former species was susceptible although mortality was low. Dead fish had significant lesions in the liver and kidney and had titers of virus greater than 108 TCID50/g. We speculate that these iridoviruses, first detected in Australia in redfin perch, are all related strains in the Ranavirus genus that may have reached these geographically distant sites by movements of ornamental fish.

Original languageEnglish (US)
Pages (from-to)423-427
Number of pages5
JournalVeterinary Research
Volume26
Issue number5-6
StatePublished - Dec 1 1995

Fingerprint

Iridovirus
Iridoviridae
ornamental fish
Fishes
perch
fish
Oncorhynchus mykiss
Perches
Poecilia reticulata
Ictalurus punctatus
viral load
viruses
Poecilia
Ictaluridae
virulence
cell lines
Ranavirus
Viruses
Viral Load
Virulence

Keywords

  • iridovirus / systemic / FV-3 / ranavirus

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Properties of iridoviruses from ornamental fish. / Hedrick, Ronald; McDowell, TS S.

In: Veterinary Research, Vol. 26, No. 5-6, 01.12.1995, p. 423-427.

Research output: Contribution to journalArticle

Hedrick, R & McDowell, TSS 1995, 'Properties of iridoviruses from ornamental fish', Veterinary Research, vol. 26, no. 5-6, pp. 423-427.
Hedrick, Ronald ; McDowell, TS S. / Properties of iridoviruses from ornamental fish. In: Veterinary Research. 1995 ; Vol. 26, No. 5-6. pp. 423-427.
@article{4eb200e34a734edc8a2a9637417205fd,
title = "Properties of iridoviruses from ornamental fish",
abstract = "An iridovirus from 2 species of freshwater tropical imported fish was examined for its antigenic and biochemical properties and virulence for 3 species of food fish. The virus was found in both guppy (Poecilia reticulata) and doctor fish (Labroides dimidatus). The virus replicated in numerous fish cell lines including BF-2, CCO and EPC. Virus titers reached 108 TCID50/ml in each cell line. The virions had similar size and morphology to those previously reported to cause systemic infections among catfish in Europe and from redfin perch and rainbow trout in Australia. A comparison of the ornamental fish iridovirus with these systemic agents indicated that all share common antigens detected by indirect fluorescent antibody tests and Western blots. Additionally, polymerase chain reactions using primer sets developed for the red fin perch virus, amplified a segment of genomic DNA from the ornamental fish isolate. An examination of the virulence of the ornamental fish isolate for 2 important food fish species, rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus), indicated the former species was susceptible although mortality was low. Dead fish had significant lesions in the liver and kidney and had titers of virus greater than 108 TCID50/g. We speculate that these iridoviruses, first detected in Australia in redfin perch, are all related strains in the Ranavirus genus that may have reached these geographically distant sites by movements of ornamental fish.",
keywords = "iridovirus / systemic / FV-3 / ranavirus",
author = "Ronald Hedrick and McDowell, {TS S.}",
year = "1995",
month = "12",
day = "1",
language = "English (US)",
volume = "26",
pages = "423--427",
journal = "Annales de Recherches Veterinaires",
issn = "0928-4249",
publisher = "BioMed Central",
number = "5-6",

}

TY - JOUR

T1 - Properties of iridoviruses from ornamental fish

AU - Hedrick, Ronald

AU - McDowell, TS S.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - An iridovirus from 2 species of freshwater tropical imported fish was examined for its antigenic and biochemical properties and virulence for 3 species of food fish. The virus was found in both guppy (Poecilia reticulata) and doctor fish (Labroides dimidatus). The virus replicated in numerous fish cell lines including BF-2, CCO and EPC. Virus titers reached 108 TCID50/ml in each cell line. The virions had similar size and morphology to those previously reported to cause systemic infections among catfish in Europe and from redfin perch and rainbow trout in Australia. A comparison of the ornamental fish iridovirus with these systemic agents indicated that all share common antigens detected by indirect fluorescent antibody tests and Western blots. Additionally, polymerase chain reactions using primer sets developed for the red fin perch virus, amplified a segment of genomic DNA from the ornamental fish isolate. An examination of the virulence of the ornamental fish isolate for 2 important food fish species, rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus), indicated the former species was susceptible although mortality was low. Dead fish had significant lesions in the liver and kidney and had titers of virus greater than 108 TCID50/g. We speculate that these iridoviruses, first detected in Australia in redfin perch, are all related strains in the Ranavirus genus that may have reached these geographically distant sites by movements of ornamental fish.

AB - An iridovirus from 2 species of freshwater tropical imported fish was examined for its antigenic and biochemical properties and virulence for 3 species of food fish. The virus was found in both guppy (Poecilia reticulata) and doctor fish (Labroides dimidatus). The virus replicated in numerous fish cell lines including BF-2, CCO and EPC. Virus titers reached 108 TCID50/ml in each cell line. The virions had similar size and morphology to those previously reported to cause systemic infections among catfish in Europe and from redfin perch and rainbow trout in Australia. A comparison of the ornamental fish iridovirus with these systemic agents indicated that all share common antigens detected by indirect fluorescent antibody tests and Western blots. Additionally, polymerase chain reactions using primer sets developed for the red fin perch virus, amplified a segment of genomic DNA from the ornamental fish isolate. An examination of the virulence of the ornamental fish isolate for 2 important food fish species, rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus), indicated the former species was susceptible although mortality was low. Dead fish had significant lesions in the liver and kidney and had titers of virus greater than 108 TCID50/g. We speculate that these iridoviruses, first detected in Australia in redfin perch, are all related strains in the Ranavirus genus that may have reached these geographically distant sites by movements of ornamental fish.

KW - iridovirus / systemic / FV-3 / ranavirus

UR - http://www.scopus.com/inward/record.url?scp=0029441791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029441791&partnerID=8YFLogxK

M3 - Article

C2 - 8581016

AN - SCOPUS:0029441791

VL - 26

SP - 423

EP - 427

JO - Annales de Recherches Veterinaires

JF - Annales de Recherches Veterinaires

SN - 0928-4249

IS - 5-6

ER -