Abstract
The unit gravity sedimentation technique was used to separate spleen cells from sevveral strains of mice. Settling patterns (plot of cell number against settling rate) were similar for BALB/c, DBA/2, C3H/He, and NZB/W mice of different ages. In particular, no subpopulation was found by this technique to be missing from the spleens of old NZB/W mice. A number of functional studies performed with the separated cells proved more informative than the settling patterns themselves. Fractions of cells which sedimented at a rate of between about 6 mm/hr and 10 mm/hr were enriched in responsiveness to PHA, Con A, and allogeneic cells. These fractions obtained from old NZB/W mice lacked such activities. However, the active fractions from young NZB/W spleens, which were enriched in θ-bearing cells, could restore the responsiveness of old NZB/W mice to primary immunization with sheep erythrocytes. These studies indicate that functional separation of spleen cells from NZB/W mice is possible and that activities lacking in whole spleens from old NZB/W mice are also lacking in the separate fractions. The ability to restore helper T cell function in old NZB/W mice with active fractions from young NZB/W mice has implications for further study and treatment of their autoimmune disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 129-142 |
| Number of pages | 14 |
| Journal | Cellular Immunology |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Immunology
Cite this
Properties of fractionated spleen cells from NZB/W mice. / Garner, George M.; Gershwin, M. Eric; Steinberg, Alfred D.
In: Cellular Immunology, Vol. 15, No. 1, 1975, p. 129-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Properties of fractionated spleen cells from NZB/W mice
AU - Garner, George M.
AU - Gershwin, M. Eric
AU - Steinberg, Alfred D.
PY - 1975
Y1 - 1975
N2 - The unit gravity sedimentation technique was used to separate spleen cells from sevveral strains of mice. Settling patterns (plot of cell number against settling rate) were similar for BALB/c, DBA/2, C3H/He, and NZB/W mice of different ages. In particular, no subpopulation was found by this technique to be missing from the spleens of old NZB/W mice. A number of functional studies performed with the separated cells proved more informative than the settling patterns themselves. Fractions of cells which sedimented at a rate of between about 6 mm/hr and 10 mm/hr were enriched in responsiveness to PHA, Con A, and allogeneic cells. These fractions obtained from old NZB/W mice lacked such activities. However, the active fractions from young NZB/W spleens, which were enriched in θ-bearing cells, could restore the responsiveness of old NZB/W mice to primary immunization with sheep erythrocytes. These studies indicate that functional separation of spleen cells from NZB/W mice is possible and that activities lacking in whole spleens from old NZB/W mice are also lacking in the separate fractions. The ability to restore helper T cell function in old NZB/W mice with active fractions from young NZB/W mice has implications for further study and treatment of their autoimmune disease.
AB - The unit gravity sedimentation technique was used to separate spleen cells from sevveral strains of mice. Settling patterns (plot of cell number against settling rate) were similar for BALB/c, DBA/2, C3H/He, and NZB/W mice of different ages. In particular, no subpopulation was found by this technique to be missing from the spleens of old NZB/W mice. A number of functional studies performed with the separated cells proved more informative than the settling patterns themselves. Fractions of cells which sedimented at a rate of between about 6 mm/hr and 10 mm/hr were enriched in responsiveness to PHA, Con A, and allogeneic cells. These fractions obtained from old NZB/W mice lacked such activities. However, the active fractions from young NZB/W spleens, which were enriched in θ-bearing cells, could restore the responsiveness of old NZB/W mice to primary immunization with sheep erythrocytes. These studies indicate that functional separation of spleen cells from NZB/W mice is possible and that activities lacking in whole spleens from old NZB/W mice are also lacking in the separate fractions. The ability to restore helper T cell function in old NZB/W mice with active fractions from young NZB/W mice has implications for further study and treatment of their autoimmune disease.
UR - http://www.scopus.com/inward/record.url?scp=0016442722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016442722&partnerID=8YFLogxK
U2 - 10.1016/0008-8749(75)90170-7
DO - 10.1016/0008-8749(75)90170-7
M3 - Article
C2 - 45836
AN - SCOPUS:0016442722
VL - 15
SP - 129
EP - 142
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -