Properties of fractionated spleen cells from NZB/W mice

George M. Garner, M. Eric Gershwin, Alfred D. Steinberg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The unit gravity sedimentation technique was used to separate spleen cells from sevveral strains of mice. Settling patterns (plot of cell number against settling rate) were similar for BALB/c, DBA/2, C3H/He, and NZB/W mice of different ages. In particular, no subpopulation was found by this technique to be missing from the spleens of old NZB/W mice. A number of functional studies performed with the separated cells proved more informative than the settling patterns themselves. Fractions of cells which sedimented at a rate of between about 6 mm/hr and 10 mm/hr were enriched in responsiveness to PHA, Con A, and allogeneic cells. These fractions obtained from old NZB/W mice lacked such activities. However, the active fractions from young NZB/W spleens, which were enriched in θ-bearing cells, could restore the responsiveness of old NZB/W mice to primary immunization with sheep erythrocytes. These studies indicate that functional separation of spleen cells from NZB/W mice is possible and that activities lacking in whole spleens from old NZB/W mice are also lacking in the separate fractions. The ability to restore helper T cell function in old NZB/W mice with active fractions from young NZB/W mice has implications for further study and treatment of their autoimmune disease.

Original languageEnglish (US)
Pages (from-to)129-142
Number of pages14
JournalCellular Immunology
Volume15
Issue number1
DOIs
StatePublished - 1975
Externally publishedYes

Fingerprint

Inbred NZB Mouse
Spleen
Cell Separation
Gravitation
Helper-Inducer T-Lymphocytes
Autoimmune Diseases
Immunization
Sheep
Cell Count
Erythrocytes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Properties of fractionated spleen cells from NZB/W mice. / Garner, George M.; Gershwin, M. Eric; Steinberg, Alfred D.

In: Cellular Immunology, Vol. 15, No. 1, 1975, p. 129-142.

Research output: Contribution to journalArticle

Garner, George M. ; Gershwin, M. Eric ; Steinberg, Alfred D. / Properties of fractionated spleen cells from NZB/W mice. In: Cellular Immunology. 1975 ; Vol. 15, No. 1. pp. 129-142.
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