Promoter-specific regulation of MyoD binding and signal transduction cooperate to pattern gene expression

Donald A. Bergstrom, Bennett Penn, Andrew Strand, Robert L.S. Perry, Michael A. Rudnicki, Stephen J. Tapscott

Research output: Contribution to journalArticle

250 Scopus citations

Abstract

We used expression arrays and chromatin immunoprecipitation assays to demonstrate that myogenesis consists of discrete subprograms of gene expression regulated by MyoD. Approximately 5% of assayed genes alter expression in a specific temporal sequence, and more than 1% are regulated by MyoD without the synthesis of additional transcription factors. MyoD regulates genes expressed at different times during myogenesis, and promoter-specific regulation of MyoD binding is a major mechanism of patterning gene expression. In addition, p38 kinase activity is necessary for the expression of a restricted subset of genes regulated by MyoD, but not for MyoD binding. The identification of distinct molecular mechanisms that regulate discrete subprograms of myogenesis should facilitate analyses of differentiation in normal development and disease.

Original languageEnglish (US)
Pages (from-to)587-600
Number of pages14
JournalMolecular Cell
Volume9
Issue number3
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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