Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus

Tara L. Walker, Ann Wierick, Alex M. Sykes, Ben Waldau, Denis Corbeil, Peter Carmeliet, Gerd Kempermann

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Prominin-1 (CD133) is commonly used to isolate stem and progenitor cells from the developing and adult nervous system and to identify cancer stem cells in brain tumors. However, despite extensive characterization of Prominin-1+ precursor cells from the adult subventricular zone, no information about the expression of Prominin-1 by precursor cells in the subgranular zone (SGZ) of the adult hippocampus has been available.Weshow here that Prominin-1 is expressed by a significant number of cells in the SGZ of adult mice in vivo and ex vivo, including postmitotic astrocytes. A small subset of Prominin-1+ cells coexpressed the nonspecific precursor cell marker Nestin as well as GFAP and Sox2. Upon fluorescence-activated cell sorting, only Prominin-1/Nestin double-positive cells fulfilled the defining stem cell criteria of proliferation, self-renewal, and multipotentiality as assessed by a neurosphere assay. In addition, isolated primary Prominin-1+cells preferentially migrated to the neurogenic niche in the SGZ upon transplantation in vivo. Finally, despite its expression by various stem and progenitor cells, Prominin-1 turned out to be dispensable for precursor cell proliferation in vitro and in vivo. Nevertheless, a net decrease in hippocampal neurogenesis, by ~30% was found in Prominin-1 knock-out mice, suggesting other roles in controlling adult hippocampal neurogenesis. Remarkably, an upregulation of Prominin-2 was detected in Prominin-1-deficient mice highlighting a potential compensatory mechanism, which might explain the lack of severe symptoms in individuals carrying mutations in the Prom1 gene.

Original languageEnglish (US)
Pages (from-to)3010-3024
Number of pages15
JournalJournal of Neuroscience
Volume33
Issue number7
DOIs
StatePublished - Feb 13 2013
Externally publishedYes

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Neural Stem Cells
Hippocampus
Stem Cells
Nestin
Neurogenesis
AC133 Antigen
Cell Proliferation
Neoplastic Stem Cells
Lateral Ventricles
Knockout Mice
Brain Neoplasms
Astrocytes
Nervous System
Flow Cytometry
Up-Regulation
Cell Count
Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Walker, T. L., Wierick, A., Sykes, A. M., Waldau, B., Corbeil, D., Carmeliet, P., & Kempermann, G. (2013). Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus. Journal of Neuroscience, 33(7), 3010-3024. https://doi.org/10.1523/JNEUROSCI.3363-12.2013

Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus. / Walker, Tara L.; Wierick, Ann; Sykes, Alex M.; Waldau, Ben; Corbeil, Denis; Carmeliet, Peter; Kempermann, Gerd.

In: Journal of Neuroscience, Vol. 33, No. 7, 13.02.2013, p. 3010-3024.

Research output: Contribution to journalArticle

Walker, TL, Wierick, A, Sykes, AM, Waldau, B, Corbeil, D, Carmeliet, P & Kempermann, G 2013, 'Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus', Journal of Neuroscience, vol. 33, no. 7, pp. 3010-3024. https://doi.org/10.1523/JNEUROSCI.3363-12.2013
Walker, Tara L. ; Wierick, Ann ; Sykes, Alex M. ; Waldau, Ben ; Corbeil, Denis ; Carmeliet, Peter ; Kempermann, Gerd. / Prominin-1 allows prospective isolation of neural stem cells from the adult murine hippocampus. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 7. pp. 3010-3024.
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