Abstract
Prolonged exercise decreased the rate of Ca+ release in sarcoplasmic reticulum (SR) vesicles isolated from rat muscle by 20-30% when release was initiated by 5, 10, and 20 μM AgNO3. [3H]Ryanodine binding was also depressed by 20% in SR vesicles isolated from the exercised animals. In contrast, the maximum amount of Ca2+ released by Ag+ remained unaffected by exercise. The passive permeability of SR vesicles and the rate of Ca2+ release in the presence of ruthenium red, a known inhibitor of the Ca2+ release mechanism, was not affected by prolonged exercise. These results suggest that exercise depressed Ca2+ release from SR by directly modifying the Ca2+ release channel.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 472-475 |
| Number of pages | 4 |
| Journal | Pflügers Archiv European Journal of Physiology |
| Volume | 422 |
| Issue number | 5 |
| DOIs | |
| State | Published - Feb 1993 |
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Keywords
- Ca release
- Exercise
- Sarcoplasmic reticulum
ASJC Scopus subject areas
- Physiology
Cite this
Prolonged exercise reduces Ca2+ release in rat skeletal muscle sarcoplasmic reticulum. / Favero, Terence G.; Pessah, Isaac N; Klug, Gary A.
In: Pflügers Archiv European Journal of Physiology, Vol. 422, No. 5, 02.1993, p. 472-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prolonged exercise reduces Ca2+ release in rat skeletal muscle sarcoplasmic reticulum
AU - Favero, Terence G.
AU - Pessah, Isaac N
AU - Klug, Gary A.
PY - 1993/2
Y1 - 1993/2
N2 - Prolonged exercise decreased the rate of Ca+ release in sarcoplasmic reticulum (SR) vesicles isolated from rat muscle by 20-30% when release was initiated by 5, 10, and 20 μM AgNO3. [3H]Ryanodine binding was also depressed by 20% in SR vesicles isolated from the exercised animals. In contrast, the maximum amount of Ca2+ released by Ag+ remained unaffected by exercise. The passive permeability of SR vesicles and the rate of Ca2+ release in the presence of ruthenium red, a known inhibitor of the Ca2+ release mechanism, was not affected by prolonged exercise. These results suggest that exercise depressed Ca2+ release from SR by directly modifying the Ca2+ release channel.
AB - Prolonged exercise decreased the rate of Ca+ release in sarcoplasmic reticulum (SR) vesicles isolated from rat muscle by 20-30% when release was initiated by 5, 10, and 20 μM AgNO3. [3H]Ryanodine binding was also depressed by 20% in SR vesicles isolated from the exercised animals. In contrast, the maximum amount of Ca2+ released by Ag+ remained unaffected by exercise. The passive permeability of SR vesicles and the rate of Ca2+ release in the presence of ruthenium red, a known inhibitor of the Ca2+ release mechanism, was not affected by prolonged exercise. These results suggest that exercise depressed Ca2+ release from SR by directly modifying the Ca2+ release channel.
KW - Ca release
KW - Exercise
KW - Sarcoplasmic reticulum
UR - http://www.scopus.com/inward/record.url?scp=0027410512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027410512&partnerID=8YFLogxK
U2 - 10.1007/BF00375074
DO - 10.1007/BF00375074
M3 - Article
C2 - 7682687
AN - SCOPUS:0027410512
VL - 422
SP - 472
EP - 475
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 5
ER -