Prolonged circulation of activated platelets following plasmapheresis

Theodore Wun, T. Paglieroni, P. Holland

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The extent and duration of in vivo platelet activation were determined in 12 volunteer donors undergoing automated plasmapheresis. Expression of P-selectin, activated GpIIb/IIIa, and platelet microparticle formation were measured by flow cytometry on peripheral blood samples obtained immediately before and after plasmapheresis and at 24 hour intervals thereafter for up to 3 days. Although no adverse effects were noted in any donor, immediately after apheresis 3-87% of circulating platelets expressed P-selectin; by 48 hours, 0.5-50% expressed P-selectin; and by 72 hours, all donors studied had fewer than 5% P-selectin expression on circulating platelets. Results were similar for the expression of the activated conformation of GpIIb/IIIa. There was a positive correlation with in vitro P-selectin expression in response to ADP in the pre-apheresis sample and the number of platelet microparticles detected in the donor following plasmapheresis. In addition, the percent expression of P-selectin and activated GpIIb/IIIa in response to ADP was reproducible in each individual studied on five separate occasions (CV ≤ 8%). Platelets activated during plasmapheresis using an automated device may circulate for at least 48 hours, and pre-plasmapheresis response of platelets to the agonist ADP correlated with platelet microparticle formation post-plasmapheresis.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalJournal of Clinical Apheresis
Volume9
Issue number1
StatePublished - 1994

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P-Selectin
Plasmapheresis
Blood Platelets
Tissue Donors
Adenosine Diphosphate
Blood Component Removal
Platelet Activation
Platelet Count
Volunteers
Flow Cytometry
Equipment and Supplies

ASJC Scopus subject areas

  • Hematology

Cite this

Prolonged circulation of activated platelets following plasmapheresis. / Wun, Theodore; Paglieroni, T.; Holland, P.

In: Journal of Clinical Apheresis, Vol. 9, No. 1, 1994, p. 10-16.

Research output: Contribution to journalArticle

Wun, Theodore ; Paglieroni, T. ; Holland, P. / Prolonged circulation of activated platelets following plasmapheresis. In: Journal of Clinical Apheresis. 1994 ; Vol. 9, No. 1. pp. 10-16.
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